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PMID: 11309422 Published · ppublish English Journal Article

Stu2 promotes mitotic spindle elongation in anaphase.

The Journal of cell biology ·Vol. 153 ·No. 2 ·2001-04-16 ·Pages 435-42

Severin F, Habermann B, Huffaker T, Hyman T

Abstract

During anaphase, mitotic spindles elongate up to five times their metaphase length. This process, known as anaphase B, is essential for correct segregation of chromosomes. Here, we examine the control of spindle length during anaphase in the budding yeast Saccharomyces cerevisiae. We show that microtubule stabilization during anaphase requires the microtubule-associated protein Stu2. We further show that the activity of Stu2 is opposed by the activity of the kinesin-related protein Kip3. Reexamination of the kinesin homology tree suggests that KIP3 is the S. cerevisiae orthologue of the microtubule-destabilizing subfamily of kinesins (Kin I). We conclude that a balance of activity between evolutionally conserved microtubule-stabilizing and microtubule-destabilizing factors is essential for correct spindle elongation during anaphase B.

MeSH Terms
Anaphase/physiology Cell Separation DNA/metabolism Flow Cytometry Fungal Proteins/metabolism Kinesins Microscopy, Fluorescence Microtubule-Associated Proteins/metabolism Phylogeny Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Spindle Apparatus/metabolism
Chemicals
Fungal Proteins KIP3 protein, S cerevisiae Microtubule-Associated Proteins STU2 protein, S cerevisiae Saccharomyces cerevisiae Proteins DNA Kinesins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Severin F
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse, 01307 Dresden, Germany.
Habermann B
Huffaker T
Hyman T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.153.2.435
Published
2001-04-16
Pages
435-42
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2169465
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040479 · United States
External Links
PubMed source DOI: 10.1083/jcb.153.2.435 PMC Full Text View journal details
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