Home LiteratureArticle Details
PMID: 11309113 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines.

Molecular microbiology ·Vol. 40 ·No. 2 ·2001-04-00 ·Pages 294-305

Christie PJ

Abstract

Bacterial conjugation systems are highly promiscuous macromolecular transfer systems that impact human health significantly. In clinical settings, conjugation is exceptionally problematic, leading to the rapid dissemination of antibiotic resistance genes and other virulence traits among bacterial populations. Recent work has shown that several pathogens of plants and mammals - Agrobacterium tumefaciens, Bordetella pertussis, Helicobacter pylori and Legionella pneumophila - have evolved secretion pathways ancestrally related to conjugation systems for the purpose of delivering effector molecules to eukaryotic target cells. Each of these systems exports distinct DNA or protein substrates to effect a myriad of changes in host cell physiology during infection. Collectively, secretion pathways ancestrally related to bacterial conjugation systems are now referred to as the type IV secretion family. The list of putative type IV family members is increasing rapidly, suggesting that macromolecular transfer by these systems is a widespread phenomenon in nature.

MeSH Terms
Bacterial Proteins/genetics,metabolism Biological Transport Conjugation, Genetic DNA, Bacterial/genetics,metabolism Eukaryotic Cells/microbiology Evolution, Molecular Gram-Negative Bacteria/metabolism,pathogenicity Gram-Negative Bacterial Infections/microbiology Humans Protein Transport Virulence
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Christie P J
Department of Microbiology and Molecular Genetics, The University of Texas-Houston Medical School, 6431 Fannin, Houston, TX 77030, USA. Peter.J.Christie@uth.tmc.edu
References (60)
60 references, click to expand
  1. The gene encoding the 17-kDa antigen of Bartonella henselae is located within a cluster of genes homologous to the virB virulence operon.
    DNA Cell Biol. 2000 Jun;19(6):377-82 PMID: 10882236
  2. The bases of crown gall tumorigenesis.
    J Bacteriol. 2000 Jul;182(14):3885-95 PMID: 10869063
  3. Involvement of a plasmid in virulence of Campylobacter jejuni 81-176.
    Infect Immun. 2000 Aug;68(8):4384-90 PMID: 10899834
  4. VirB6 is required for stabilization of VirB5 and VirB3 and formation of VirB7 homodimers in Agrobacterium tumefaciens.
    J Bacteriol. 2000 Aug;182(16):4505-11 PMID: 10913084
  5. Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells.
    Trends Microbiol. 2000 Aug;8(8):354-60 PMID: 10920394
  6. The T-pilus of Agrobacterium tumefaciens.
    Trends Microbiol. 2000 Aug;8(8):361-9 PMID: 10920395
  7. A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication.
    J Bacteriol. 2000 Sep;182(17):4849-55 PMID: 10940027
  8. Rhizobium etli CE3 carries vir gene homologs on a self-transmissible plasmid.
    Mol Plant Microbe Interact. 2000 Sep;13(9):1019-21 PMID: 10975659
  9. Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob.
    Mol Microbiol. 2000 Sep;37(6):1283-92 PMID: 10998162
  10. Helicobacter pylori inhibits phagocytosis by professional phagocytes involving type IV secretion components.
    Mol Microbiol. 2000 Sep;37(6):1389-404 PMID: 10998171
  11. VirB/D4-dependent protein translocation from Agrobacterium into plant cells.
    Science. 2000 Nov 3;290(5493):979-82 PMID: 11062129
  12. DNA-independent transport of plasmid primase protein between bacteria by the I1 conjugation system.
    Mol Microbiol. 2000 Nov;38(3):650-7 PMID: 11069687
  13. Maturation of IncP pilin precursors resembles the catalytic Dyad-like mechanism of leader peptidases.
    J Bacteriol. 2000 Dec;182(23):6751-61 PMID: 11073921
  14. Formation of a fibrous structure on the surface of Legionella pneumophila associated with exposure of DotH and DotO proteins after intracellular growth.
    Mol Microbiol. 2001 Jan;39(2):313-29 PMID: 11136453
  15. Crystal structure of the hexameric traffic ATPase of the Helicobacter pylori type IV secretion system.
    Mol Cell. 2000 Dec;6(6):1461-72 PMID: 11163218
  16. Genetic transformation of HeLa cells by Agrobacterium.
    Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1871-6 PMID: 11172043
  17. An Agrobacterium VirE2 channel for transferred-DNA transport into plant cells.
    Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):485-90 PMID: 11149937
  18. Biology of type II secretion.
    Mol Microbiol. 2001 Apr;40(2):271-83 PMID: 11309111
  19. Type III export: new uses for an old pathway.
    Mol Microbiol. 2001 Apr;40(2):284-93 PMID: 11309112
  20. Two-partner secretion in Gram-negative bacteria: a thrifty, specific pathway for large virulence proteins.
    Mol Microbiol. 2001 Apr;40(2):306-13 PMID: 11309114
  21. Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4.
    Mol Microbiol. 1990 Jul;4(7):1199-205 PMID: 2172695
  22. Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria.
    J Bacteriol. 1997 May;179(10):3085-94 PMID: 9150199
  23. Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation.
    Mol Gen Genet. 1997 Apr 28;254(4):400-6 PMID: 9180693
  24. TrwD, a protein encoded by the IncW plasmid R388, displays an ATP hydrolase activity essential for bacterial conjugation.
    J Biol Chem. 1997 Oct 10;272(41):25583-90 PMID: 9325277
  25. Construction of transposon Tn3phoA: its application in defining the membrane topology of the Agrobacterium tumefaciens DNA transfer proteins.
    Mol Microbiol. 1998 Jan;27(2):405-14 PMID: 9484895
  26. The Escherichia coli ATP-binding cassette (ABC) proteins.
    Mol Microbiol. 1998 Apr;28(1):5-13 PMID: 9593292
  27. The carboxyl terminus of protein TraD adds specificity and efficiency to F-plasmid conjugative transfer.
    J Bacteriol. 1998 Nov;180(22):6039-42 PMID: 9811665
  28. IncP plasmids are unusually effective in mediating conjugation of Escherichia coli and Saccharomyces cerevisiae: involvement of the tra2 mating system.
    J Bacteriol. 1998 Dec;180(24):6538-43 PMID: 9851996
  29. Biochemistry of type IV secretion.
    Curr Opin Microbiol. 1999 Feb;2(1):25-9 PMID: 10047550
  30. Cell biology of Legionella pneumophila.
    Curr Opin Microbiol. 1999 Feb;2(1):30-4 PMID: 10047559
  31. VirE1 is a specific molecular chaperone for the exported single-stranded-DNA-binding protein VirE2 in Agrobacterium.
    Mol Microbiol. 1999 Mar;31(6):1795-807 PMID: 10209751
  32. Helicobacter pylori virulence and genetic geography.
    Science. 1999 May 21;284(5418):1328-33 PMID: 10334982
  33. Genetic evidence of protein transfer during bacterial conjugation.
    Plasmid. 1999 May;41(3):240-7 PMID: 10366529
  34. Dimerization of the Agrobacterium tumefaciens VirB4 ATPase and the effect of ATP-binding cassette mutations on the assembly and function of the T-DNA transporter.
    Mol Microbiol. 1999 Jun;32(6):1239-53 PMID: 10383764
  35. Mutagenesis of the Agrobacterium VirE2 single-stranded DNA-binding protein identifies regions required for self-association and interaction with VirE1 and a permissive site for hybrid protein construction.
    J Bacteriol. 1999 Jul;181(14):4342-52 PMID: 10400593
  36. Conjugative pili of IncP plasmids, and the Ti plasmid T pilus are composed of cyclic subunits.
    J Biol Chem. 1999 Aug 6;274(32):22548-55 PMID: 10428832
  37. Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.
    J Bacteriol. 1999 Oct;181(19):6108-13 PMID: 10498725
  38. A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis.
    Mol Microbiol. 1999 Sep;33(6):1210-20 PMID: 10510235
  39. Mutants in the ptlA-H genes of Bordetella pertussis are deficient for pertussis toxin secretion.
    FEMS Microbiol Lett. 1999 Oct 15;179(2):479-84 PMID: 10518754
  40. The Agrobacterium tumefaciens chaperone-like protein, VirE1, interacts with VirE2 at domains required for single-stranded DNA binding and cooperative interaction.
    J Bacteriol. 1999 Nov;181(21):6850-5 PMID: 10542192
  41. Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila.
    Mol Microbiol. 1999 Nov;34(4):799-809 PMID: 10564519
  42. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori.
    Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14559-64 PMID: 10588744
  43. Characterization of ATP and DNA binding activities of TrwB, the coupling protein essential in plasmid R388 conjugation.
    J Biol Chem. 1999 Dec 17;274(51):36117-24 PMID: 10593894
  44. The Agrobacterium T-DNA transport pore proteins VirB8, VirB9, and VirB10 interact with one another.
    J Bacteriol. 2000 Feb;182(3):758-63 PMID: 10633111
  45. Modulation of Helicobacter pylori induced interleukin-8 synthesis in gastric epithelial cells mediated by cag PAI encoded VirD4 homologue.
    J Clin Pathol. 1999 Sep;52(9):653-7 PMID: 10655985
  46. Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation.
    Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1263-8 PMID: 10655519
  47. Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis.
    Infect Immun. 2000 Mar;68(3):1297-303 PMID: 10678941
  48. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion.
    Science. 2000 Feb 25;287(5457):1497-500 PMID: 10688800
  49. TraG from RP4 and TraG and VirD4 from Ti plasmids confer relaxosome specificity to the conjugal transfer system of pTiC58.
    J Bacteriol. 2000 Mar;182(6):1541-8 PMID: 10692358
  50. Sequence-related protein export NTPases encoded by the conjugative transfer region of RP4 and by the cag pathogenicity island of Helicobacter pylori share similar hexameric ring structures.
    Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3067-72 PMID: 10716714
  51. Cloning, sequencing, and expression of three Bartonella henselae genes homologous to the Agrobacterium tumefaciens VirB region.
    DNA Cell Biol. 2000 Mar;19(3):141-7 PMID: 10749166
  52. Conjugative junctions in RP4-mediated mating of Escherichia coli.
    J Bacteriol. 2000 May;182(10):2709-15 PMID: 10781537
  53. Enzymology of type IV macromolecule secretion systems: the conjugative transfer regions of plasmids RP4 and R388 and the cag pathogenicity island of Helicobacter pylori encode structurally and functionally related nucleoside triphosphate hydrolases.
    J Bacteriol. 2000 May;182(10):2761-70 PMID: 10781544
  54. Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore.
    Mol Microbiol. 2000 May;36(3):608-17 PMID: 10844650
  55. Genetic and environmental factors affecting T-pilin export and T-pilus biogenesis in relation to flagellation of Agrobacterium tumefaciens.
    J Bacteriol. 2000 Jul;182(13):3705-16 PMID: 10850985
  56. The N- and C-terminal portions of the Agrobacterium VirB1 protein independently enhance tumorigenesis.
    J Bacteriol. 2000 Jun;182(12):3437-45 PMID: 10852875
  57. Importance of holotoxin assembly in Ptl-mediated secretion of pertussis toxin from Bordetella pertussis.
    Infect Immun. 2000 Jul;68(7):4049-54 PMID: 10858221
  58. Identification of genes required for chronic persistence of Brucella abortus in mice.
    Infect Immun. 2000 Jul;68(7):4102-7 PMID: 10858227
  59. Transferred DNA (T-DNA)-associated proteins of Agrobacterium tumefaciens are exported independently of virB.
    Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7545-50 PMID: 10852952
  60. Self-assembly of the Agrobacterium tumefaciens VirB11 traffic ATPase.
    J Bacteriol. 2000 Aug;182(15):4137-45 PMID: 10894719
Article Info
Journal
Molecular microbiology
Abbr.
Mol Microbiol
ISSN
0950-382X
DOI
10.1046/j.1365-2958.2001.02302.x
Email
peter.j.christie@uth.tmc.edu
Published
2001-04-00
Pages
294-305
Language
English
Region
England
NLM ID
8712028
PMCID
PMC3922410
Subset
IM
Grants
NIGMS NIH HHS · R01 GM048746 · United States
NIGMS NIH HHS · GM48746 · United States
External Links
PubMed source DOI: 10.1046/j.1365-2958.2001.02302.x PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2