Home LiteratureArticle Details
PMID: 10858221 Published · ppublish English Journal Article

Importance of holotoxin assembly in Ptl-mediated secretion of pertussis toxin from Bordetella pertussis.

Infection and immunity ·Vol. 68 ·No. 7 ·2000-07-00 ·Pages 4049-54

Farizo KM, Huang T, Burns DL

Abstract

We examined the structural components of pertussis toxin that are required for efficient export from Bordetella pertussis via the Ptl system, a member of the type IV family of macromolecular transporters. First, we constructed a strain of B. pertussis that contains a functional Ptl system but does not produce pertussis toxin. Plasmids which express either the S1 subunit or the B oligomer were then introduced into this strain. We found that the B oligomer of the toxin is not secreted in the absence of the S1 subunit. Conversely, the S1 subunit is also not secreted by a Ptl-mediated mechanism in the absence of the B oligomer. Thus, an assembled holotoxin is required for Ptl-mediated export of pertussis toxin from B. pertussis.

MeSH Terms
Base Sequence Biological Transport, Active Bordetella pertussis/genetics,metabolism,pathogenicity DNA Primers/genetics Genes, Bacterial Pertussis Toxin Plasmids/genetics Protein Structure, Quaternary Sequence Deletion Virulence Virulence Factors, Bordetella/chemistry,genetics,metabolism
Chemicals
DNA Primers Virulence Factors, Bordetella Pertussis Toxin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Farizo K M
Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA. farizo@cber.fda.gov
Huang T
Burns D L
References (31)
31 references, click to expand
  1. Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis.
    J Bacteriol. 1991 Jul;173(14):4288-96 PMID: 2066330
  2. Bordetella pertussis adenylate cyclase toxin and hemolytic activities require a second gene, cyaC, for activation.
    J Bacteriol. 1991 Jan;173(2):720-6 PMID: 1987161
  3. Gene-for-genes interactions between cotton R genes and Xanthomonas campestris pv. malvacearum avr genes.
    Mol Plant Microbe Interact. 1993 Mar-Apr;6(2):225-37 PMID: 8097122
  4. Use of conditionally counterselectable suicide vectors for allelic exchange.
    Methods Enzymol. 1994;235:458-65 PMID: 8057916
  5. Detection and subcellular localization of three Ptl proteins involved in the secretion of pertussis toxin from Bordetella pertussis.
    J Bacteriol. 1994 Sep;176(17):5350-6 PMID: 8071211
  6. The crystal structure of pertussis toxin.
    Structure. 1994 Jan 15;2(1):45-57 PMID: 8075982
  7. Adaptation of a conjugal transfer system for the export of pathogenic macromolecules.
    Trends Microbiol. 1996 Feb;4(2):64-8 PMID: 8820569
  8. Analysis of proteins encoded by the ptx and ptl genes of Bordetella bronchiseptica and Bordetella parapertussis.
    Infect Immun. 1996 Oct;64(10):4020-6 PMID: 8926063
  9. Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria.
    J Bacteriol. 1997 May;179(10):3085-94 PMID: 9150199
  10. Essential role of the consensus nucleotide-binding site of PtlH in secretion of pertussis toxin from Bordetella pertussis.
    J Bacteriol. 1997 Dec;179(23):7577-80 PMID: 9393726
  11. Identification and characterization of PtlC, an essential component of the pertussis toxin secretion system.
    Infect Immun. 1999 Feb;67(2):754-9 PMID: 9916087
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  13. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.
    J Mol Biol. 1975 Aug 5;96(2):307-16 PMID: 1100846
  14. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  15. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  16. Direct modification of the membrane adenylate cyclase system by islet-activating protein due to ADP-ribosylation of a membrane protein.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3129-33 PMID: 6954463
  17. Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model.
    Biochemistry. 1982 Oct 26;21(22):5516-22 PMID: 6293544
  18. The A protomer of islet-activating protein, pertussis toxin, as an active peptide catalyzing ADP-ribosylation of a membrane protein.
    Arch Biochem Biophys. 1983 Jul 1;224(1):290-8 PMID: 6683482
  19. Interaction of monoclonal antibodies with pertussis toxin and its subunits.
    Infect Immun. 1984 Oct;46(1):195-201 PMID: 6207108
  20. Pertussis toxin gene: nucleotide sequence and genetic organization.
    Science. 1986 Jun 6;232(4755):1258-64 PMID: 3704651
  21. Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4631-5 PMID: 2873570
  22. G proteins: transducers of receptor-generated signals.
    Annu Rev Biochem. 1987;56:615-49 PMID: 3113327
  23. Structural characterization of pertussis toxin A subunit.
    J Biol Chem. 1987 Dec 25;262(36):17677-82 PMID: 3320046
  24. Epitopes on the S1 subunit of pertussis toxin recognized by monoclonal antibodies.
    Infect Immun. 1989 Mar;57(3):944-50 PMID: 2465274
  25. Monoclonal antibodies to pertussis toxin: utilization as probes of toxin function.
    Hybridoma. 1989 Feb;8(1):37-51 PMID: 2466764
  26. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.
    Gene. 1988 Oct 15;70(1):191-7 PMID: 2853689
  27. Use of the promoter fusion transposon Tn5 lac to identify mutations in Bordetella pertussis vir-regulated genes.
    Infect Immun. 1989 Sep;57(9):2674-82 PMID: 2569447
  28. Formation of ion channels by colicin B in planar lipid bilayers.
    J Membr Biol. 1990 Mar;114(1):79-95 PMID: 1690810
  29. Roles of the disulfide bond and the carboxy-terminal region of the S1 subunit in the assembly and biosynthesis of pertussis toxin.
    Infect Immun. 1990 Jun;58(6):1518-26 PMID: 2341166
  30. The subunit S1 is important for pertussis toxin secretion.
    J Biol Chem. 1990 Oct 15;265(29):17759-63 PMID: 2211659
  31. Molecular characterization of an operon required for pertussis toxin secretion.
    Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2970-4 PMID: 8464913
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.7.4049-4054.2000
Email
farizo@cber.fda.gov
Published
2000-07-00
Pages
4049-54
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC101693
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.68.7.4049-4054.2000 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2