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PMID: 11272899 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Multiplex genotype analysis of invasive carcinoma and accompanying proliferative lesions microdissected from breast tissue.

The Journal of molecular diagnostics : JMD ·Vol. 2 ·No. 1 ·2000-02-00 ·Pages 29-36

Cui X, Feiner H, Lin Z, Li H

Abstract

To understand the genetic basis of breast cancer in a comprehensive way, purported precursor lesions need to be analyzed at a large number of genetic marker loci and compared with each other and with the invasive components. However, the microscopic size of most of these lesions and the very small amount of material that can be obtained through microdissection limit the number of loci that can be included in the analysis. To address this issue, a multiplex genotyping approach has been developed. With this approach, polymorphic sequences at 28 marker loci were amplified simultaneously from the micro-dissected components in 5-microm paraffin-embedded breast tissue sections. The genotypes of the lesions were determined after resolving the amplified allelic products by denaturing gradient gel electrophoresis. Because the material isolated from each lesion in a single 5-microm section was sufficient for several 28-locus assays and several successive tissue sections with the same set of lesions may be prepared, it is possible to determine the genotype of each lesion at hundreds of genetic marker loci that may well cover the human genome. Analyzing a sufficient number of cases may yield information that could be used to understand the genetic basis of breast cancer development in a comprehensive way.

MeSH Terms
Alleles Biomarkers, Tumor/genetics Breast Neoplasms/genetics,pathology Female Genotype Humans Neoplasm Invasiveness Polymerase Chain Reaction
Chemicals
Biomarkers, Tumor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cui X
Coriell Institute for Medical Research, Camden, New Jersey 08103, USA.
Feiner H
Lin Z
Li H
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43 references, click to expand
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Article Info
Journal
The Journal of molecular diagnostics : JMD
Abbr.
J Mol Diagn
ISSN
1525-1578
DOI
10.1016/S1525-1578(10)60612-5
Published
2000-02-00
Pages
29-36
Language
English
Region
United States
NLM ID
100893612
PMCID
PMC1906894
Subset
IM
Grants
NCI NIH HHS · CA77363 · United States
External Links
PubMed source DOI: 10.1016/S1525-1578(10)60612-5 PMC Full Text View journal details
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