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PMID: 11265758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TIF-IA, the factor mediating growth-dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p.

EMBO reports ·Vol. 1 ·No. 2 ·2000-08-00 ·Pages 171-5

Bodem J, Dobreva G, Hoffmann-Rohrer U, Iben S, Zentgraf H, Delius H, Vingron M, Grummt I

Abstract

Cells carefully modulate the rate of rRNA transcription in order to prevent an overinvestment in ribosome synthesis under less favorable nutritional conditions. In mammals, growth-dependent regulation of RNA polymerase I (Pol I) transcription is mediated by TIF-IA, an essential initiation factor that is active in extracts from growing but not starved or cycloheximide-treated mammalian cells. Here we report the molecular cloning and functional characterization of recombinant TIF-IA, which turns out to be the mammalian homolog of the yeast factor Rrn3p. We demonstrate that TIF-IA interacts with Pol I in the absence of template DNA, augments Pol I transcription in vivo and rescues transcription in extracts from growth-arrested cells in vitro.

MeSH Terms

3T3 Cells Amino Acid Sequence Animals Blotting, Western Cloning, Molecular Genes, Reporter/genetics HeLa Cells Humans Mice Microscopy, Fluorescence Molecular Sequence Data Plasmids/genetics,metabolism Pol1 Transcription Initiation Complex Proteins RNA, Ribosomal/biosynthesis,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Alignment Transcription Factors/chemistry,genetics,metabolism Transfection Yeasts/genetics

Chemicals

Pol1 Transcription Initiation Complex Proteins RNA, Ribosomal RRN3 protein, S cerevisiae RRN3 protein, human Rrn3 protein, mouse Saccharomyces cerevisiae Proteins Transcription Factors

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Bodem J

Division of Molecular Biology of the Cell II, German Cancer Research Center, Heidelberg.

Dobreva G

Hoffmann-Rohrer U

Iben S

Zentgraf H

Delius H

Vingron M

Grummt I

References (13)

13 references, click to expand

RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human.
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4724-9 PMID: 10758157
The rapid turnover of RNA polymerase of rat liver nucleolus, and of its messenger RNA.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2833-7 PMID: 4507607
Growth-dependent regulation of rRNA synthesis is mediated by a transcription initiation factor (TIF-IA).
Nucleic Acids Res. 1985 Nov 25;13(22):8165-80 PMID: 4070001
Transcription of mouse rDNA is regulated by an activated subform of RNA polymerase I.
Cell. 1987 Sep 11;50(6):873-83 PMID: 3621348
A growth-dependent transcription initiation factor (TIF-IA) interacting with RNA polymerase I regulates mouse ribosomal RNA synthesis.
EMBO J. 1990 Sep;9(9):2857-63 PMID: 2390974
Hormonal regulation of transcription of rDNA. Purification and characterization of the hormone-regulated transcription factor IC.
J Biol Chem. 1990 Sep 25;265(27):16225-33 PMID: 2398050
p53 represses ribosomal gene transcription.
Oncogene. 1999 Jan 28;18(4):1119-24 PMID: 10023689
Factor C*, the specific initiation component of the mouse RNA polymerase I holoenzyme, is inactivated early in the transcription process.
Mol Cell Biol. 1994 Jul;14(7):5010-21 PMID: 8007994
RRN3 gene of Saccharomyces cerevisiae encodes an essential RNA polymerase I transcription factor which interacts with the polymerase independently of DNA template.
EMBO J. 1996 Aug 1;15(15):3964-73 PMID: 8670901
Purification, assay, and properties of RNA polymerase I and class I-specific transcription factors in mouse.
Methods Enzymol. 1996;273:233-48 PMID: 8791616
A specialized form of RNA polymerase I, essential for initiation and growth-dependent regulation of rRNA synthesis, is disrupted during transcription.
EMBO J. 1998 Jul 1;17(13):3692-703 PMID: 9649439
Regulation of mammalian ribosomal gene transcription by RNA polymerase I.
Prog Nucleic Acid Res Mol Biol. 1999;62:109-54 PMID: 9932453
Function of the growth-regulated transcription initiation factor TIF-IA in initiation complex formation at the murine ribosomal gene promoter.
Mol Cell Biol. 1993 Nov;13(11):6723-32 PMID: 8413268

Article Info

Journal

EMBO reports

Abbr.

EMBO Rep

ISSN

1469-221X

DOI

10.1093/embo-reports/kvd032

Published

2000-08-00

Pages

171-5

Language

English

Region

England

NLM ID

100963049

PMCID

PMC1084264

Subset

Databases

GENBANK

AJ272050

External Links

PubMed source DOI: 10.1093/embo-reports/kvd032 PMC Full Text View journal details

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TIF-IA, the factor mediating growth-dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p.

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