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PMID: 11222601 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of the varR gene as a transcriptional regulator of virginiamycin S resistance in Streptomyces virginiae.

Journal of bacteriology ·Vol. 183 ·No. 6 ·2001-03-00 ·Pages 2025-31

Namwat W, Lee CK, Kinoshita H, Yamada Y, Nihira T

Abstract

A gene designated varR (for virginiae antibiotic resistance regulator) was identified in Streptomyces virginiae 89 bp downstream of a varS gene encoding a virginiamycin S (VS)-specific transporter. The deduced varR product showed high homology to repressors of the TetR family with a conserved helix-turn-helix DNA binding motif. Purified recombinant VarR protein was present as a dimer in vitro and showed clear DNA binding activity toward the varS promoter region. This binding was abolished by the presence of VS, suggesting that VarR regulates transcription of varS in a VS-dependent manner. Northern blot analysis revealed that varR was cotranscribed with upstream varS as a 2.4-kb transcript and that VS acted as an inducer of bicistronic transcription. Deletion analysis of the varS promoter region clarified two adjacent VarR binding sites in the varS promoter.

MeSH Terms
Amino Acid Sequence Bacterial Proteins Base Sequence Binding Sites DNA-Binding Proteins/chemistry,genetics,metabolism Drug Resistance, Microbial/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Membrane Proteins/genetics,metabolism Molecular Sequence Data Promoter Regions, Genetic Recombinant Proteins/isolation & purification,metabolism Repressor Proteins/chemistry,genetics,metabolism Sequence Analysis, DNA Streptomyces/drug effects,genetics,growth & development,metabolism Transcription, Genetic Virginiamycin/pharmacology
Chemicals
Bacterial Proteins DNA-Binding Proteins Membrane Proteins Recombinant Proteins Repressor Proteins varR protein, Streptomyces virginiae varS protein, Streptomyces virginiae Virginiamycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Namwat W
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Lee C K
Kinoshita H
Yamada Y
Nihira T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.6.2025-2031.2001
Published
2001-03-00
Pages
2025-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95098
Subset
IM
Databases
GENBANK
AB046994
External Links
PubMed source DOI: 10.1128/JB.183.6.2025-2031.2001 PMC Full Text View journal details
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