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PMID: 11179370 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Role of alginate O acetylation in resistance of mucoid Pseudomonas aeruginosa to opsonic phagocytosis.

Infection and immunity ·Vol. 69 ·No. 3 ·2001-03-00 ·Pages 1895-901

Pier GB, Coleman F, Grout M, Franklin M, Ohman DE

Abstract

Establishment and maintenance of chronic lung infections with mucoid Pseudomonas aeruginosa in patients with cystic fibrosis (CF) require that the bacteria avoid host defenses. Elaboration of the extracellular, O-acetylated mucoid exopolysaccharide, or alginate, is a major microbial factor in resistance to immune effectors. Here we show that O acetylation of alginate maximizes the resistance of mucoid P. aeruginosa to antibody-independent opsonic killing and is the molecular basis for the resistance of mucoid P. aeruginosa to normally nonopsonic but alginate-specific antibodies found in normal human sera and sera of infected CF patients. O acetylation of alginate appears to be critical for P. aeruginosa resistance to host immune effectors in CF patients.

MeSH Terms
Acetylation Alginates Bacterial Capsules Complement Activation Complement Pathway, Alternative Cystic Fibrosis/complications Humans Lung Diseases/complications,etiology Opsonin Proteins Phagocytosis Pseudomonas Infections/complications,etiology Pseudomonas aeruginosa/pathogenicity
Chemicals
Alginates Opsonin Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pier G B
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115-5804, USA. gpier@channing.harvard.edu
Coleman F
Grout M
Franklin M
Ohman D E
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.69.3.1895-1901.2001
Email
gpier@channing.harvard.edu
Published
2001-03-00
Pages
1895-901
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC98099
Subset
IM
Grants
NIAID NIH HHS · R01 AI046588 · United States
NIAID NIH HHS · AI 19146 · United States
NIAID NIH HHS · AI 22836 · United States
NIAID NIH HHS · R56 AI019146 · United States
NIAID NIH HHS · AI46588 · United States
NIAID NIH HHS · R01 AI019146 · United States
External Links
PubMed source DOI: 10.1128/IAI.69.3.1895-1901.2001 PMC Full Text View journal details
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