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PMID: 11179226 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An active role for a structured B-linker in effector control of the sigma54-dependent regulator DmpR.

The EMBO journal ·Vol. 20 ·No. 4 ·2001-02-15 ·Pages 819-27

O'Neill E, Wikström P, Shingler V

Abstract

The activities of many prokaryotic sigma54-dependent transcriptional activators are controlled by the N-terminal A-domain of the protein, which is linked to the central transcriptional activation domain via a short B-linker. It used to be thought that these B-linkers simply serve as flexible tethers. Here we show that the B-linker of the aromatic-responsive regulator DmpR and many other regulators of the family contain signature heptad repeats with regularly spaced hydrophobic amino acids. Mutant analysis of this region of DmpR demonstrates that B-linker function is dependent on the heptad repeats and is critical for activation of the protein by aromatic effectors. The phenotypes of DmpR mutants refute the existing model that the level of ATPase activity directly controls the level of transcription it promotes. The mutant analysis also shows that the B-linker is involved in repression of ATPase activity and that allosteric changes upon effector binding are transduced to alleviate both B-linker repression of ATP hydrolysis and A-domain repression of transcriptional activation. The mechanistic implications of these findings for DmpR and other family members are discussed.

MeSH Terms
Adenosine Triphosphatases/metabolism Amino Acid Sequence Bacterial Proteins/metabolism DNA-Binding Proteins DNA-Directed RNA Polymerases/metabolism Molecular Sequence Data RNA Polymerase Sigma 54 Repetitive Sequences, Amino Acid Sequence Homology, Amino Acid Sigma Factor/metabolism Trans-Activators/metabolism Transcriptional Activation
Chemicals
Bacterial Proteins DNA-Binding Proteins DmpR protein, Pseudomonas Sigma Factor Trans-Activators DNA-Directed RNA Polymerases RNA Polymerase Sigma 54 Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
O'Neill E
Department of Cell and Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
Wikström P
Shingler V
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.4.819
Published
2001-02-15
Pages
819-27
Language
English
Region
England
NLM ID
8208664
PMCID
PMC145425
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.4.819 PMC Full Text View journal details
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