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PMID: 10629187 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HbpR, a new member of the XylR/DmpR subclass within the NtrC family of bacterial transcriptional activators, regulates expression of 2-hydroxybiphenyl metabolism in Pseudomonas azelaica HBP1.

Journal of bacteriology ·Vol. 182 ·No. 2 ·2000-01-00 ·Pages 405-17

Jaspers MC, Suske WA, Schmid A, Goslings DA, Kohler HP, van der Meer JR

Abstract

The regulation of 2-hydroxybiphenyl and 2,2'-dihydroxybiphenyl degradation in Pseudomonas azelaica is mediated by the regulatory gene, hbpR. The hbpR gene encodes a 63-kDa protein belonging to the NtrC family of prokaryotic transcriptional activators and having the highest homology to members of the XylR/DmpR subclass. Disruption of the hbpR gene in P. azelaica and complementation in trans showed that the HbpR protein was the key regulator for 2-hydroxybiphenyl metabolism. Induction experiments with P. azelaica and Escherichia coli containing luxAB-based transcriptional fusions revealed that HbpR activates transcription from a promoter (P(hbpC)) in front of the first gene for 2-hydroxybiphenyl degradation, hbpC, and that 2-hydroxybiphenyl itself is the direct effector for HbpR-mediated activation. Of several compounds tested, only the pathway substrates 2-hydroxybiphenyl and 2,2'-dihydroxybiphenyl and structural analogs like 2-aminobiphenyl and 2-hydroxybiphenylmethane were effectors for HbpR activation. HbpR is therefore, to our knowledge, the first regulator of the XylR/DmpR class that recognizes biaromatic but not monoaromatic structures. Analysis of a spontaneously occurring mutant, P. azelaica HBP1 Prp, which can grow with the non-wild-type effector 2-propylphenol, revealed a single mutation in the hbpR gene (T613C) leading to a Trp-->Arg substitution at amino acid residue 205. P. azelaica HBP1 derivative strains without a functional hbpR gene constitutively expressed the genes for 2-hydroxybiphenyl degradation when complemented in trans with the hbpR-T613C gene. This suggests the importance of this residue, which is conserved among all members of the XylR/DmpR subclass, for interdomain repression.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Biphenyl Compounds/metabolism DNA-Binding Proteins/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Molecular Sequence Data Mutation PII Nitrogen Regulatory Proteins Phenols/metabolism Pseudomonas/genetics Sequence Alignment Sequence Analysis, DNA Trans-Activators/genetics Transcription Factors/genetics
Chemicals
Bacterial Proteins Biphenyl Compounds DNA-Binding Proteins Escherichia coli Proteins HbpR protein, Pseudomonas azelaica PII Nitrogen Regulatory Proteins Phenols Trans-Activators Transcription Factors glnG protein, E coli 2-propylphenol 2-phenylphenol
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jaspers M C
Swiss Federal Institute for Environmental Science and Technology, CH-8600 Dübendorf, Switzerland.
Suske W A
Schmid A
Goslings D A
Kohler H P
van der Meer J R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.2.405-417.2000
Published
2000-01-00
Pages
405-17
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94290
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.182.2.405-417.2000 PMC Full Text View journal details
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