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PMID: 11178260 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Can sequence determine function?

Genome biology ·Vol. 1 ·No. 5 ·2000-00-00 ·Pages REVIEWS0005

Gerlt JA, Babbitt PC

Abstract

The functional annotation of proteins identified in genome sequencing projects is based on similarities to homologs in the databases. As a result of the possible strategies for divergent evolution, homologous enzymes frequently do not catalyze the same reaction, and we conclude that assignment of function from sequence information alone should be viewed with some skepticism.

MeSH Terms
Animals Binding Sites/genetics DNA/chemistry,genetics Enzymes/genetics,metabolism Genome Humans Sequence Alignment Sequence Analysis, DNA Substrate Specificity
Chemicals
Enzymes DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gerlt J A
Departments of Biochemistry and Chemistry, University of Illinois, Urbana, IL 61801, USA. j-gerlt@uiuc.edu
Babbitt P C
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Article Info
Journal
Genome biology
Abbr.
Genome Biol
ISSN
1474-760X
DOI
10.1186/gb-2000-1-5-reviews0005
Email
j-gerlt@uiuc.edu
Published
2000-00-00
Epub
2000-00-08
Pages
REVIEWS0005
Language
English
Region
England
NLM ID
100960660
PMCID
PMC138884
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058506 · United States
NIGMS NIH HHS · GM-52594 · United States
NIGMS NIH HHS · GM-58506 · United States
NIGMS NIH HHS · R01 GM052594 · United States
NIGMS NIH HHS · R01 GM060595 · United States
NIGMS NIH HHS · GM-60595 · United States
NIGMS NIH HHS · GM-40570 · United States
External Links
PubMed source DOI: 10.1186/gb-2000-1-5-reviews0005 PMC Full Text View journal details
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