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PMID: 11158311 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gadd153 sensitizes cells to endoplasmic reticulum stress by down-regulating Bcl2 and perturbing the cellular redox state.

Molecular and cellular biology ·Vol. 21 ·No. 4 ·2001-02-00 ·Pages 1249-59

McCullough KD, Martindale JL, Klotz LO, Aw TY, Holbrook NJ

Abstract

gadd153, also known as chop, is a highly stress-inducible gene that is robustly expressed following disruption of homeostasis in the endoplasmic reticulum (ER) (so-called ER stress). Although all reported types of ER stress induce expression of Gadd153, its role in the stress response has remained largely undefined. Several studies have correlated Gadd153 expression with cell death, but a mechanistic link between Gadd153 and apoptosis has never been demonstrated. To address this issue we employed a cell model system in which Gadd153 is constitutively overexpressed, as well as two cell lines in which Gadd153 expression is conditional. In all cell lines, overexpression of Gadd153 sensitized cells to ER stress. Investigation of the mechanisms contributing to this effect revealed that elevated Gadd153 expression results in the down-regulation of Bcl2 expression, depletion of cellular glutathione, and exaggerated production of reactive oxygen species. Restoration of Bcl2 expression in Gadd153-overexpressing cells led to replenishment of glutathione and a reduction in levels of reactive oxygen species, and it protected cells from ER stress-induced cell death. We conclude that Gadd153 sensitizes cells to ER stress through mechanisms that involve down-regulation of Bcl2 and enhanced oxidant injury.

MeSH Terms
Animals Base Sequence CCAAT-Enhancer-Binding Proteins/genetics,metabolism Cell Line DNA Primers/genetics Down-Regulation Endoplasmic Reticulum/metabolism Gene Expression Genes, bcl-2 Glutathione/metabolism HeLa Cells Humans Mice Mutagenesis, Site-Directed Oxidation-Reduction Oxidative Stress Proto-Oncogene Proteins c-bcl-2/genetics,metabolism Transcription Factor CHOP Transcription Factors/genetics,metabolism
Chemicals
CCAAT-Enhancer-Binding Proteins DDIT3 protein, human DNA Primers Ddit3 protein, mouse Proto-Oncogene Proteins c-bcl-2 Transcription Factors Transcription Factor CHOP Glutathione
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McCullough K D
Cell Stress and Aging Section, Laboratory of Biological Chemistry, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224-6825, USA.
Martindale J L
Klotz L O
Aw T Y
Holbrook N J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
Published
2001-02-00
Pages
1249-59
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC99578
Subset
IM
Grants
NIDDK NIH HHS · R01 DK044510 · United States
NIDDK NIH HHS · R01 DK044510-08 · United States
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