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PMID: 11121473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of human DNA topoisomerase I with G-quartet structures.

Nucleic acids research ·Vol. 28 ·No. 24 ·2000-12-15 ·Pages 4832-8

Arimondo PB, Riou JF, Mergny JL, Tazi J, Sun JS, Garestier T, Hélène C

Abstract

Because of their role in the control of the topological state of DNA, topoisomerases are ubiquitous and vital enzymes, which participate in nearly all events related to DNA metabolism including replication and transcription. We show here that human topoisomerase I (Topo I) plays an unexpected role of 'molecular matchmaker' for G-quartet formation. G-quadruplexes are multi-stranded structures held together by square planes of four guanines ('G-quartets') interacting by forming Hoogsteen hydrogen bonds. Topo I is able to promote the formation of four-stranded intermolecular DNA structures when added to single-stranded DNA containing a stretch of at least five guanines. We provide evidence that these complexes are parallel G-quartet structures, mediated by tetrads of hydrogen-bonded guanine. In addition, Topo I binds specifically to pre-formed parallel and anti-parallel G4-DNA.

MeSH Terms
Base Sequence Binding Sites DNA/chemistry,genetics,metabolism DNA Probes/chemistry,genetics,metabolism DNA Topoisomerases, Type I/metabolism DNA, Viral/chemistry,genetics,metabolism DNA-Binding Proteins/metabolism Guanine/chemistry,metabolism HIV-1/genetics Humans Hydrogen Bonding Models, Molecular Nucleic Acid Conformation Oligodeoxyribonucleotides/chemistry,genetics,metabolism Protein Binding
Chemicals
DNA Probes DNA, Viral DNA-Binding Proteins Oligodeoxyribonucleotides Guanine DNA DNA Topoisomerases, Type I
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Arimondo P B
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle CNRS UMR 8646, INSERM U201, 43 rue Cuvier, 75231 Paris cedex 05, France.
Riou J F
Mergny J L
Tazi J
Sun J S
Garestier T
Hélène C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/28.24.4832
Published
2000-12-15
Pages
4832-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC115246
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/28.24.4832 PMC Full Text View journal details
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