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PMID: 11105762 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dual role for the RNA-binding domain of Xenopus laevis SLBP1 in histone pre-mRNA processing.

RNA (New York, N.Y.) ·Vol. 6 ·No. 11 ·2000-11-00 ·Pages 1635-48

Ingledue TC, Dominski Z, Sánchez R, Erkmann JA, Marzluff WF

Abstract

The replication-dependent histone mRNAs end in a conserved 26-nt sequence that forms a stem-loop structure. This sequence is required for histone pre-mRNA processing and plays a role in multiple aspects of histone mRNA metabolism. Two proteins that bind the 3' end of histone mRNA are found in Xenopus oocytes. xSLBP1 is found in the nucleus, where it functions in histone pre-mRNA processing, and in the cytoplasm, where it may control histone mRNA translation and stability. xSLBP2 is a cytoplasmic protein, inactive in histone pre-mRNA processing, whose expression is restricted to oogenesis and early development. These proteins are similar only in their RNA-binding domains (RBD). A chimeric protein (1-2-1) in which the RBD of xSLBP1 has been replaced with the RBD of xSLBP2 binds the stem-loop with an affinity similar to the original protein. The 1-2-1 protein efficiently localizes to the nucleus of the frog oocyte, but is not active in processing of histone pre-mRNA in vivo. This protein does not support processing in a nuclear extract, but inhibits processing by competing with the active SLBP by binding to the substrate. The 1-2-1 protein also inhibits processing of synthetic histone pre-mRNA injected into frog oocytes, but has no effect on processing of histone pre-mRNA transcribed from an injected histone gene. This result suggests that sequences in the RBD of xSLBP1 give it preferential access to histone pre-mRNA transcribed in vivo.

MeSH Terms
Amino Acid Sequence Animals Binding Sites Female Histones/genetics Molecular Sequence Data Nuclear Proteins Oocytes/physiology RNA Precursors/genetics,metabolism RNA Processing, Post-Transcriptional RNA, Messenger/genetics RNA-Binding Proteins/chemistry,genetics,metabolism Recombinant Fusion Proteins/biosynthesis Sequence Alignment Sequence Homology, Amino Acid Xenopus Proteins Xenopus laevis mRNA Cleavage and Polyadenylation Factors
Chemicals
Histones Nuclear Proteins RNA Precursors RNA, Messenger RNA-Binding Proteins Recombinant Fusion Proteins SLBP1 protein, Xenopus Xenopus Proteins mRNA Cleavage and Polyadenylation Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ingledue T C
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599, USA.
Dominski Z
Sánchez R
Erkmann J A
Marzluff W F
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838200000819
Published
2000-11-00
Pages
1635-48
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370032
Subset
IM
Grants
NIGMS NIH HHS · GM29832 · United States
NIGMS NIH HHS · GM58921 · United States
NIGMS NIH HHS · T32GM08570 · United States
External Links
PubMed source DOI: 10.1017/s1355838200000819 PMC Full Text View journal details
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