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PMID: 11102367 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutations affecting the development of the peripheral nervous system in Drosophila: a molecular screen for novel proteins.

Genetics ·Vol. 156 ·No. 4 ·2000-12-00 ·Pages 1691-715

Prokopenko SN, He Y, Lu Y, Bellen HJ

Abstract

In our quest for novel genes required for the development of the embryonic peripheral nervous system (PNS), we have performed three genetic screens using MAb 22C10 as a marker of terminally differentiated neurons. A total of 66 essential genes required for normal PNS development were identified, including 49 novel genes. To obtain information about the molecular nature of these genes, we decided to complement our genetic screens with a molecular screen. From transposon-tagged mutations identified on the basis of their phenotype in the PNS we selected 31 P-element strains representing 26 complementation groups on the second and third chromosomes to clone and sequence the corresponding genes. We used plasmid rescue to isolate and sequence 51 genomic fragments flanking the sites of these P-element insertions. Database searches using sequences derived from the ends of plasmid rescues allowed us to assign genes to one of four classes: (1) previously characterized genes (11), (2) first mutations in cloned genes (1), (3) P-element insertions in genes that were identified, but not characterized molecularly (1), and (4) novel genes (13). Here, we report the cloning, sequence, Northern analysis, and the embryonic expression pattern of candidate cDNAs for 10 genes: astray, chrowded, dalmatian, gluon, hoi-polloi, melted, pebble, skittles, sticky ch1, and vegetable. This study allows us to draw conclusions about the identity of proteins required for the development of the nervous system in Drosophila and provides an example of a molecular approach to characterize en masse transposon-tagged mutations identified in genetic screens.

MeSH Terms
Animals Cell Differentiation Cloning, Molecular DNA Transposable Elements/genetics DNA, Complementary/genetics Drosophila melanogaster/embryology,genetics Embryo, Nonmammalian/metabolism Gene Expression Profiling Genes, Insect Genetic Complementation Test In Situ Hybridization Insect Proteins/genetics,physiology Mutagenesis, Insertional Nerve Tissue Proteins/genetics,physiology Neurons/chemistry Peripheral Nervous System/embryology
Chemicals
DNA Transposable Elements DNA, Complementary Insect Proteins Nerve Tissue Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Prokopenko S N
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA. prokopenko@salk.edu
He Y
Lu Y
Bellen H J
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/156.4.1691
Email
prokopenko@salk.edu
Published
2000-12-00
Pages
1691-715
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461357
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/156.4.1691 PMC Full Text View journal details
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