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PMID: 11078509 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 24 ·2000-11-21 ·Pages 13401-6

Llave C, Kasschau KD, Carrington JC

Abstract

Certain plant viruses encode suppressors of posttranscriptional gene silencing (PTGS), an adaptive antiviral defense response that limits virus replication and spread. The tobacco etch potyvirus protein, helper component-proteinase (HC-Pro), suppresses PTGS of silenced transgenes. The effect of HC-Pro on different steps of the silencing pathway was analyzed by using both transient Agrobacterium tumefaciens-based delivery and transgenic systems. HC-Pro inactivated PTGS in plants containing a preexisting silenced beta-glucuronidase (GUS) transgene. PTGS in this system was associated with both small RNA molecules (21-26 nt) corresponding to the 3' proximal region of the transcribed GUS sequence and cytosine methylation of specific sites near the 3' end of the GUS transgene. Introduction of HC-Pro into these plants resulted in loss of PTGS, loss of small RNAs, and partial loss of methylation. These results suggest that HC-Pro targets a PTGS maintenance (as opposed to an initiation or signaling) component at a point that affects accumulation of small RNAs and methylation of genomic DNA.

MeSH Terms
Agrobacterium tumefaciens/genetics,growth & development Crosses, Genetic Cysteine Endopeptidases/genetics,metabolism Gene Silencing Genetic Vectors Glucuronidase/genetics Homozygote Plants, Genetically Modified Plants, Toxic Potyvirus/genetics,pathogenicity,physiology RNA, Plant/genetics Suppression, Genetic Tobacco/genetics,virology Viral Proteins/genetics,metabolism Virus Replication
Chemicals
RNA, Plant Viral Proteins Glucuronidase Cysteine Endopeptidases HC-Pro protein, potyvirus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Llave C
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
Kasschau K D
Carrington J C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.230334397
Published
2000-11-21
Pages
13401-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC27236
Subset
IM
Grants
NIAID NIH HHS · AI 27832 · United States
NIAID NIH HHS · R37 AI043288 · United States
NIAID NIH HHS · AI 43288 · United States
NIAID NIH HHS · R21 AI043288 · United States
NIAID NIH HHS · R01 AI043288 · United States
External Links
PubMed source DOI: 10.1073/pnas.230334397 PMC Full Text View journal details
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