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PMID: 1107798 Published · ppublish English Journal Article

The stoichiometry of the ribosomal proteins of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 140 ·No. 3 ·1975-10-03 ·Pages 253-74

Hardy SJ

Abstract

A ribosome preparation from E. coli made without stringent washing procedures has been shown to contain the same relative amounts of nearly all the ribosomal proteins as ribosomes in intact cells. Stoichiometric measurements on all the proteins of this preparation except for L8, L20, L31 and L34 have been made using an isotope dilution technique. When the scatter of the values obtained, the uncertainty in the molecular weights, and the losses occurring during extraction are taken into account, none of the proteins except L7/L12 is present at a level significantly different from one molecule per ribosome. There are multiple copies of L7/L12. These data suggest that the ribosomes of Escherichia coli are homogeneous in vivo.

MeSH Terms
Bacterial Proteins/analysis Electrophoresis Escherichia coli Molecular Weight Ribosomal Proteins/analysis
Chemicals
Bacterial Proteins Ribosomal Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hardy S J
References (25)
25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334270
Published
1975-10-03
Pages
253-74
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334270
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