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PMID: 110683 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Surface macromolecules and virulence in intracellular parasitism: comparison of cell envelope components of smooth and rough strains of Brucella abortus.

Infection and immunity ·Vol. 23 ·No. 3 ·1979-03-00 ·Pages 819-28

Kreutzer DL, Robertson DC

Abstract

The surface topography of whole cells and the chemical composition of cell envelopes of a smooth-intermediate strain (45/0) and a rough strain (45/20) of Brucella abortus was examined. Electron microscopy of whole cells and thin sections did not reveal any gross surface difference(s). Only minor quantitative differences were observed in total lipids, proteins, and the murein layer. However, the lipopolysaccharide composition of the two strains was quite different. Both phenol- and water-soluble lipopolysaccharide fractions were obtained from the strain of higher virulence (45/0), whereas only aqueous lipopolysaccharide could be isolated from the rough strain. In addition to being toxic, the phenol-soluble lipopolysaccharide may be a key virulence factor in intracellular survival of B. obortus within phagocytic cells.

MeSH Terms
Bacterial Proteins/analysis Brucella abortus/analysis,ultrastructure Cell Wall/analysis Hydrolysis Lipids/analysis Lipopolysaccharides/analysis Lipoproteins/analysis Macromolecular Substances Muramidase/metabolism Peptidoglycan/analysis Trypsin/metabolism
Chemicals
Bacterial Proteins Lipids Lipopolysaccharides Lipoproteins Macromolecular Substances Peptidoglycan Muramidase Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kreutzer D L
Robertson D C
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38 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.23.3.819-828.1979
Published
1979-03-00
Pages
819-28
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414238
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.23.3.819-828.1979 PMC Full Text View journal details
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