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PMID: 5669888 Published · ppublish English Journal Article

Carbohydrate composition of the phenol-soluble lipopolysaccharides of Citrobacter freundii.

Journal of bacteriology ·Vol. 95 ·No. 6 ·1968-06-00 ·Pages 2035-43

Raff RA, Wheat RW

Abstract

Phenol-soluble lipopolysaccharides were obtained from the interphase and phenol phase fractions of 44% aqueous phenol-extracted Citrobacter species. Upon detailed investigation of C. freundii 8090, the two lipopolysaccharide fractions were found to contain different amounts of lipid A, although qualitative composition was similar. Both contained lipid A, 2-keto-deoxyoctonic acid, heptose, phosphate, d-glucose, galactose, rhamnose, 2-acetamido-2 deoxy-d-glucose, 3-acetamido-3,6-dideoxy-d-glucose, O-acetyl, and trace amino acids. Partially purified phenol-phase lipopolysaccharide partitioned into the phenol-soluble phase when refractionated with 44% aqueous phenol, and was further found to be soluble in 88% phenol, 95% ethyl alcohol, and chloroform-methanol (2:1).

MeSH Terms
Amino Acids/analysis Amino Sugars/analysis Carbohydrates/analysis Chemistry Techniques, Analytical Chromatography, Gas Chromatography, Thin Layer Escherichia/analysis Glucosamine/analysis Glucose/analysis Lipopolysaccharides/analysis Phenols
Chemicals
Amino Acids Amino Sugars Carbohydrates Lipopolysaccharides Phenols Glucose Glucosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Raff R A
Wheat R W
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.95.6.2035-2043.1968
Published
1968-06-00
Pages
2035-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC315131
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.95.6.2035-2043.1968 PMC Full Text View journal details
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