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PMID: 11060077 Published · ppublish English Comparative Study Evaluation Study Journal Article

Species-specific identification of human adenoviruses by a multiplex PCR assay.

Journal of clinical microbiology ·Vol. 38 ·No. 11 ·2000-11-00 ·Pages 4114-20

Xu W, McDonough MC, Erdman DD

Abstract

A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.

MeSH Terms
Adenovirus Infections, Human/virology Adenoviruses, Human/classification,genetics,isolation & purification Capsid/genetics Capsid Proteins DNA Primers/genetics Humans Polymerase Chain Reaction/methods Reproducibility of Results Sensitivity and Specificity Species Specificity
Chemicals
Capsid Proteins DNA Primers hexon capsid protein, Adenovirus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xu W
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
McDonough M C
Erdman D D
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.38.11.4114-4120.2000
Published
2000-11-00
Pages
4114-20
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC87550
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/JCM.38.11.4114-4120.2000 PMC Full Text View journal details
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