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PMID: 11042117 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase.

The Biochemical journal ·Vol. 351 Pt 3 ·2000-11-01 ·Pages 629-37

Yauch RL, Hemler ME

Abstract

In earlier work we established that phosphoinositide 4-kinase (PI 4-kinase) may associate with transmembrane 4 superfamily (TM4SF, tetraspanin) proteins, but critical specificity issues were not addressed. Here we demonstrate that at least five different TM4SF proteins (CD9, CD63, CD81, CD151 and A15/TALLA1) can associate with a similar or identical 55 kDa type II PI 4-kinase. These associations were specific, since we found no evidence for other phosphoinositide kinases (e.g. phosphoinositide 3-kinase and phosphoinositide-4-phosphate 5-kinase) associating with TM4SF proteins, and many other TM4SF proteins (including CD82 and CD53) did not associate with PI 4-kinase. CD63-PI 4-kinase complexes were almost entirely intracellular, and thus are distinct from other TM4SF-PI 4-kinase complexes (e.g. involving CD9), which are largely located in the plasma membrane. These results suggest that a specific subset of TM4SF proteins may recruit PI 4-kinase to specific membrane locations, and thereby influence phosphoinositide-dependent signalling.

MeSH Terms
1-Phosphatidylinositol 4-Kinase/chemistry,metabolism Amino Acid Sequence Blotting, Western Cell Line Electrophoresis, Polyacrylamide Gel Humans Membrane Proteins/metabolism Molecular Sequence Data Precipitin Tests Protein Binding Substrate Specificity
Chemicals
Membrane Proteins 1-Phosphatidylinositol 4-Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yauch R L
Dana-Farber Cancer Institute, Rm D-1430, 44 Binney Street, Boston, MA 02115, USA.
Hemler M E
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
2000-11-01
Pages
629-37
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1221402
Subset
IM
Grants
NIGMS NIH HHS · GM38903 · United States
External Links
PubMed source PMC Full Text View journal details
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