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PMID: 8688552 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of novel complexes on the cell surface between integrins and proteins with 4 transmembrane domains (TM4 proteins).

Molecular biology of the cell ·Vol. 7 ·No. 2 ·1996-02-00 ·Pages 193-207

Berditchevski F, Zutter MM, Hemler ME

Abstract

Here we identified several new integrin/TM4 protein complexes on the cell surface. By immunoprecipitation using nonstringent conditions, and by reciprocal immunoprecipitation, we found that alpha 3 beta 1 and alpha 6 beta 1 integrins but not alpha 2 beta 1, alpha 5 beta 1, or alpha 6 beta 4 integrins associated with CD9 and CD81 in alpha 3 beta 1/CD81, alpha 3 beta 1/CD9, alpha 6 beta 1/CD81, and alpha 6 beta 1/CD9 complexes. Also, cross-linking experiments established that alpha 3 beta 1/CD81, alpha 3 beta 1/CD9, and alpha 3 beta 1/CD63 associations occur on the surface of intact cells and suggested that a critical interaction site is located within extracellular domains. Cross-linking in conjunction with reimmunoprecipitation indicated that larger multi-component alpha 3 beta 1/TM4/TM4 complexes (alpha 3 beta 1/CD9/CD63, alpha 3 beta 1/CD81/CD63, and alpha 3 beta 1/CD9/CD81) also could be detected on the cell surface. Immunofluorescent staining showed redistribution of alpha 3 beta 1/TM4 complexes toward the periphery of cells plated on various extracellular matrix substrates and also showed that these complexes were localized in cell footprints. Staining of human tissues yielded additional results consistent with co-localization of alpha 3 beta 1 and CD9, CD63, and CD81 proteins. In conclusion we suggest that the prevalence of integrin/TM4 complexes in diverse cellular environments is indicative of their general physiological importance.

MeSH Terms
Antigens, CD/metabolism Antigens, Surface/metabolism Cell Line Cell Membrane/metabolism HeLa Cells Humans Integrin alpha3beta1 Integrin alpha6beta1 Integrin alpha6beta4 Integrins/metabolism Membrane Glycoproteins Membrane Proteins Platelet Membrane Glycoproteins/metabolism Protein Binding Tetraspanin 28 Tetraspanin 29 Tetraspanin 30 Tumor Cells, Cultured
Chemicals
Antigens, CD Antigens, Surface CD63 protein, human CD81 protein, human CD9 protein, human Integrin alpha3beta1 Integrin alpha6beta1 Integrin alpha6beta4 Integrins Membrane Glycoproteins Membrane Proteins Platelet Membrane Glycoproteins Tetraspanin 28 Tetraspanin 29 Tetraspanin 30
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berditchevski F
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Zutter M M
Hemler M E
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.7.2.193
Published
1996-02-00
Pages
193-207
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275873
Subset
IM
Grants
NIGMS NIH HHS · GM-38903 · United States
External Links
PubMed source DOI: 10.1091/mbc.7.2.193 PMC Full Text View journal details
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