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PMID: 1103139 Published · ppublish English Journal Article

Cleavage of adenovirus messenger RNA and of 28S and 18S ribosomal RNA by RNase III.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 8 ·1975-08-00 ·Pages 3077-81

Westphal H, Crouch RJ

Abstract

Escherichia coli ribonuclease III cleaves adenovirus messenger RNA and mammalian 28S and 18S ribosomal RNA. Fragmentation is not random, but in each case a specific collection of products is generated. This points to the potential use of the enzyme as a tool for specific fragmentation of RNA. Cleavage by RNase III abolishes the capability of adenovirus messenger RNA to direct cell-free synthesis of virus polypeptides.

MeSH Terms
Adenoviridae/analysis Cell Line Escherichia coli/enzymology,metabolism Protein Biosynthesis RNA, Messenger RNA, Ribosomal RNA, Viral Ribonucleases/metabolism
Chemicals
RNA, Messenger RNA, Ribosomal RNA, Viral Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Westphal H
Crouch R J
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.8.3077
Published
1975-08-00
Pages
3077-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432923
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.8.3077 PMC Full Text View journal details
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