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PMID: 4346034 Published · ppublish English Journal Article

Double-stranded RNA as an inhibitor of protein synthesis and as a substrate for a nuclease in extracts of Krebs II ascites cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 225-9

Robertson HD, Mathews MB

Abstract

Concentrations of double-stranded RNA above about 0.1 mug/ml inhibit translation of encephalo-myocarditis viral RNA and mouse globin messenger RNA in extracts of Krebs II ascites cells. Protein synthesis initially proceeds at the control rate, then abruptly shuts off in a manner similar to that observed in reticulocyte lysates [Hunt, T. & Ehrenfeld, E. (1971) Nature New Biol. 230, 91-94]. Substantially higher concentrations of double-stranded RNA are required to give this effect in ascites extracts. Subcellular fractions of Krebs II ascites cells contain a nucleolytic activity capable of digesting several natural and synthetic double-stranded RNAs. This nuclease is most active under conditions of protein synthesis, and part of the activity remains associated with ribosomes upon sedimentation. It is probably because of digestion of double-stranded RNA by this nuclease that higher concentrations of double-stranded RNA are required for inhibition of protein synthesis in Krebs cell extracts than in reticulocyte lysates.

MeSH Terms
Animals Bacteriophages/analysis Carbon Isotopes Carcinoma, Krebs 2/enzymology,metabolism Cell-Free System Encephalomyocarditis virus/metabolism Escherichia coli/enzymology Globins/biosynthesis Kinetics Mice Neoplasm Proteins/biosynthesis Phosphorus Isotopes Protein Biosynthesis/drug effects RNA, Messenger/metabolism RNA, Viral/isolation & purification,metabolism,pharmacology Reoviridae/analysis Ribonucleases/metabolism
Chemicals
Carbon Isotopes Neoplasm Proteins Phosphorus Isotopes RNA, Messenger RNA, Viral Globins Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Robertson H D
Mathews M B
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.1.225
Published
1973-01-00
Pages
225-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433220
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.1.225 PMC Full Text View journal details
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