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PMID: 1102933 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcriptional control of the isoleucine-valine messenger RNA's in E. coli K-12.

Molecular & general genetics : MGG ·Vol. 138 ·No. 3 ·1975-06-19 ·Pages 257-68

Childs GJ, Freundlich M

Abstract

Hybridization of messenger ribonucleic acid (mRNA) isolated from Escherichia Coli K-12 to deoxyribonucleic acid (DNA) from lambdaCI857st68h80dilv was used to detect isoleucine-valine (ilv) specific mRNA. A number of strains partially constitutive for the isoleucine-valine enzymes had levels of ilv mRNA 2 to 3-fold higher than the parent strain. Starvation for any of the branched-chain amino acids resulted in a 20 to 23-fold increase in ilv mRNA as compared to repressed levels. These differences were not due to altered growth rates or to changes in the stability of ilv mRNA. These data indicate that regulation of the isoleucine-valine enzymes by multivalent repression occurs mainly at the level of transcription. Kinetics of elongation of ilv mRNA after repression are consistent with the assumption that the mechanism of multivalent repression involves the prevention of further initiations by RNA polymerase.

MeSH Terms
DNA Escherichia coli/metabolism Isoleucine/metabolism Mutation Nucleic Acid Hybridization Operon RNA, Bacterial/metabolism RNA, Messenger/metabolism Valine/metabolism
Chemicals
RNA, Bacterial RNA, Messenger Isoleucine DNA Valine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Childs G J
Freundlich M
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269352
Published
1975-06-19
Pages
257-68
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00269352
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