Home LiteratureArticle Details
PMID: 1102932 Published · ppublish English Journal Article

Pyrimidine biosynthesis in Aspergillus nidulans: isolation and preliminary characterisation of auxotrophic mutants.

Molecular & general genetics : MGG ·Vol. 138 ·No. 3 ·1975-06-19 ·Pages 243-55

Palmer LM, Cove DJ

Abstract

113 pyrimidine auxotrophs, unable to synthesise UMP have been selected in Aspergillus nidulans. These mutants can be classified by complementation into eight groups, and genetic analysis has shown that five loci are involved. One complex locus consists of the mutually complementing pyrA, pyrB and pyrC groups, as well as the cis-dominant pyrN group, members of which do not complement with members of the A, B or C groups. pyrA mutants have been shown to lack CPSase-ur, pyrB and pyrC mutants have been shown to lack ACTase, and pyrN to lack both these enzymes. This locus appears to code for products which form an enzyme aggregate. The four simple loci, as well as the complex loci have been located genetically, and distinguished from one another on the basis of accumulation of pyrimidine precursors in vivo. The synthesis of ACTase has been shown to subject to end-product repression.

MeSH Terms
Aspartate Carbamoyltransferase/analysis Aspergillus nidulans/enzymology,metabolism Chromosome Mapping Chromosomes, Bacterial Crosses, Genetic Genetic Complementation Test Genetic Linkage Mutation Pyrimidines/biosynthesis
Chemicals
Pyrimidines Aspartate Carbamoyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Palmer L M
Cove D J
References (23)
23 references, click to expand
  1. Production of heterozygous diploids in filamentous fungi.
    Experientia. 1952 Jan 15;8(1):14-5 PMID: 14945382
  2. Suppressor of pyrimidine 3 mutants of Neurospora and its relation to arginine synthesis.
    Science. 1961 Aug 18;134(3477):470-1 PMID: 13720237
  3. The genetics of Aspergillus nidulans.
    Adv Genet. 1953;5:141-238 PMID: 13040135
  4. Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1968 Mar;95(3):824-32 PMID: 5651325
  5. [The fine structure of the ur-l locus in Coprinus radiatus].
    Mol Gen Genet. 1969 Oct 13;105(2):164-81 PMID: 5392190
  6. Aggregation states of a regulatory enzyme complex catalyzing the early steps of pyrimidine biosynthesis in bakers' yeast.
    Can J Biochem. 1971 Apr;49(4):403-11 PMID: 5552824
  7. The biosynthesis of carbamoyl phosphate in Saccharomyces cerevisiae.
    J Gen Microbiol. 1965 Jul;40(1):127-42 PMID: 5856369
  8. The enzymology of control by feedback inhibition.
    J Biol Chem. 1962 Mar;237:891-6 PMID: 13897943
  9. Evolutionary significance of autogenous regulation.
    Nature. 1974 Sep 20;251(5472):256 PMID: 4609342
  10. Pyrimidine synthesis in Neurospora crassa: gene-enzyme relationships.
    J Bacteriol. 1969 Dec;100(3):1371-7 PMID: 5361218
  11. Polarised complementation at the pyrimidine-3 locus of Neurospora.
    Mol Gen Genet. 1969 Jul 3;104(3):288-94 PMID: 5364467
  12. The aspartate transcarbamylase and carbamoyl phosphate synthetase of yeast: a multi-functional enzyme complex.
    Biochem Biophys Res Commun. 1969 Feb 21;34(4):426-33 PMID: 5776390
  13. Fine structure of the ura 2 locus in Saccharomyces cervisiae. I. In vivo complementation studies.
    Mol Gen Genet. 1971;112(4):354-64 PMID: 5129798
  14. Heat-induced disaggregation of a multifunctional enzyme complex catalyzing the first steps in pyrimidine biosynthesis in bakers' yeast.
    Can J Biochem. 1970 Feb;48(2):155-9 PMID: 5417412
  15. The induction and repression of nitrate reductase in the fungus Aspergillus nidulans.
    Biochim Biophys Acta. 1966 Jan 11;113(1):51-6 PMID: 5940632
  16. Autogenous regulation of gene expression.
    Science. 1974 Mar 1;183(4127):810-6 PMID: 4589900
  17. Genetic analyses of the pyr-3 locus of Neurospora crassa: the bearing of recombination and gene conversion upon intraallelic linearity.
    Genetica. 1959;30:293-311 PMID: 13835862
  18. Carbamyl phosphate synthesis in Neurospora crassa. I. Preliminary characterization of arginine-specific carbamyl phosphokinase.
    Biochim Biophys Acta. 1965 Aug 24;107(1):44-53 PMID: 5857367
  19. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  20. Pyrimidine-specific carbamyl phosphate synthetase in Neurospora crassa.
    J Bacteriol. 1970 Aug;103(2):335-41 PMID: 5432004
  21. Pyrimidine-requiring suppressor mutations of arginine-3 in Neurospora and their bearing on the structure of the pyrimidine-3 locus.
    Mol Gen Genet. 1970;107(2):97-106 PMID: 5520151
  22. The use of p-fluorophenylalanine with 'master strains' of Aspergillus nidulans for assigning genes to linkage groups.
    Genet Res. 1965 Nov;6(3):352-9 PMID: 5848714
  23. Pyrimidine metabolism in microorganisms.
    Bacteriol Rev. 1970 Sep;34(3):278-343 PMID: 4919542
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269351
Published
1975-06-19
Pages
243-55
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00269351
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2