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PMID: 11014803 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Suppressors of mdm20 in yeast identify new alleles of ACT1 and TPM1 predicted to enhance actin-tropomyosin interactions.

Genetics ·Vol. 156 ·No. 2 ·2000-10-00 ·Pages 523-34

Singer JM, Hermann GJ, Shaw JM

Abstract

The actin cytoskeleton is required for many aspects of cell division in yeast, including mitochondrial partitioning into growing buds (mitochondrial inheritance). Yeast cells lacking MDM20 function display defects in both mitochondrial inheritance and actin organization, specifically, a lack of visible actin cables and enhanced sensitivity to Latrunculin A. mdm20 mutants also exhibit a temperature-sensitive growth phenotype, which we exploited to isolate second-site suppressor mutations. Nine dominant suppressors selected in an mdm20/mdm20 background rescue temperature-sensitive growth defects and mitochondrial inheritance defects and partially restore actin cables in haploid and diploid mdm20 strains. The suppressor mutations define new alleles of ACT1 and TPM1, which encode actin and the major form of tropomyosin in yeast, respectively. The ACT1 mutations cluster in a region of the actin protein predicted to contact tropomyosin, suggesting that they stabilize actin cables by enhancing actin-tropomyosin interactions. The characteristics of the mutant ACT1 and TPM1 alleles and their potential effects on protein structure and binding are discussed.

MeSH Terms
Acetyltransferases Actins/chemistry,genetics,metabolism Alleles Binding Sites Bridged Bicyclo Compounds, Heterocyclic/pharmacology Fungal Proteins/chemistry,genetics,metabolism Genes, Fungal Genotype Models, Molecular Mutagenesis Mutagenesis, Site-Directed N-Terminal Acetyltransferase B Protein Conformation Saccharomyces cerevisiae/drug effects,genetics,growth & development Saccharomyces cerevisiae Proteins Suppression, Genetic Temperature Thiazoles/pharmacology Thiazolidines Tropomyosin/chemistry,genetics,metabolism
Chemicals
Actins Bridged Bicyclo Compounds, Heterocyclic Fungal Proteins MDM20 protein, S cerevisiae Saccharomyces cerevisiae Proteins Thiazoles Thiazolidines Tropomyosin Acetyltransferases N-Terminal Acetyltransferase B latrunculin A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Singer J M
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
Hermann G J
Shaw J M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/156.2.523
Published
2000-10-00
Pages
523-34
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461267
Subset
IM
Grants
NIGMS NIH HHS · GM53466 · United States
NIGMS NIH HHS · T32GM07464 · United States
External Links
PubMed source DOI: 10.1093/genetics/156.2.523 PMC Full Text View journal details
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