Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle.

Radiation and environmental biophysics ·Vol. 12 ·No. 1 ·1975-06-13 ·Pages 31-9

Baisch H, Göhde W, Linden WA

Abstract

Mathematical models for the analysis of pulse-cytophotometric (PCP) data are described. With computer programs based on these models the fractions of cells in G1-, S- and (G2 + M)-phase are obtained. The methods are applied to PCP-measurements of Ehrlich ascites tumor cells, human bone marrow cells and L-929-cells in culture. The results of the L-cell experiment are compared with autoradiographic results; for both methods the duration of the various phases has been calculated. Two different mathematical models for PCP-data evaluation and the autoradiographic method yielded results agreeing within statistical error. The application of the two models on different types of DNA-histograms is discussed: One model is suitable for asynchronous cell populations with a low fraction of S-phase cells, the other can be applied for partially synchronized cells and high S-phase fractions as well.

MeSH Terms

Animals Bone Marrow Cells Carcinoma, Ehrlich Tumor/metabolism,pathology Cell Division Cytological Techniques DNA/biosynthesis Humans L Cells/cytology,metabolism Mathematics Pulse Radiolysis

Chemicals

DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Baisch H

Göhde W

Linden W A

References (12)

12 references, click to expand

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Article Info

Journal

Radiation and environmental biophysics

Abbr.

Radiat Environ Biophys

ISSN

0301-634X

DOI

10.1007/BF02339807

Published

1975-06-13

Pages

31-9

Language

English

Region

Germany

NLM ID

0415677

Subset

External Links

PubMed source DOI: 10.1007/BF02339807 View journal details

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Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle.

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