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PMID: 1101267 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A bacterial mutation blocking P2 phage late gene expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2770-4

Sunshine MG, Sauer B

Abstract

A mutant of Escherichia coli strain C has been isolated, called gro109, that blocks bacteriophage P2 propagation by interfering with late gene expression. DNA replication proceeds normally in P2+-infected gro109 cells, but late phage proteins are not made. Early P2 mRNA is made in normal amounts, but very little late mRNA can be detected. P2 mutants (P2 ogr) able to overcome the gro109 block have been isolated in which synthesis of late P2 mRNA and phage proteins Is restored. The gro109 mutation is closely linked to the cluster of ribosomal genes at 64 min and is recessive to the wild-type (gro+) allele. A P2 ogr mutation has been mapped on the left arm of the P2 genome, between the right-most known late gene (D) and the phage attachment site. P2 ogr can complement P2+ in gro109 cells, indicating that ogr codes for a diffusible product.

MeSH Terms
Coliphages/metabolism Crosses, Genetic DNA, Viral/biosynthesis Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Genes, Dominant Genetic Complementation Test Genotype Hybridization, Genetic Mutation Recombination, Genetic Species Specificity Thymidine/metabolism Time Factors Transduction, Genetic Viral Plaque Assay
Chemicals
DNA, Viral Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sunshine M G
Sauer B
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2770
Published
1975-07-00
Pages
2770-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432853
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2770 PMC Full Text View journal details
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