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PMID: 109813 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Initiation of translation with Pseudomonas aeruginosa phage PP7 RNA: nucleotide sequence of the coat cistron ribosome binding site.

Nucleic acids research ·Vol. 6 ·No. 5 ·1979-00-00 ·Pages 2003-16

Bassel BA, Mills DR

Abstract

Initiation complex formation between PP7 RNA and ribosomes of Pseudomonas aeruginosa and Escherichia coli has been investigated. The PP7 RNA fragments protected by both species of ribosome have been isolated, and their sequences have been determined. Only one binding sites is available on the intact PP7 RNA strand, and this site is recognized by ribosomes of both species. The PP7 RNA binding site is approximately 38 nucleotides long. It contains two AUG sequences and a purine-rich segment near the 5'-end that is complementary to segments near the 3'-ends of the 16S ribosomal RNA's of both P. aeruginosa and E. coli. In order to establish which of the AUG codons acts as the initiator, the H2N-terminal amino acid sequence of PP7 coat protein was determined. This sequence is compatible with the codon sequence following the second AUG codon. The extent of the reaction of PP7 RNA with E. coli ribosomes is greater than with P. aeruginosa ribosomes, but our results do not indicate a qualitative difference in the initial interaction between intact PP7 RNA and the ribosomes of either species.

MeSH Terms
Amino Acid Sequence Bacteriophages/metabolism Base Sequence Codon Escherichia coli/metabolism Peptide Chain Initiation, Translational Peptide Initiation Factors Protein Biosynthesis Pseudomonas aeruginosa/metabolism RNA, Ribosomal/metabolism RNA, Viral/metabolism Ribosomes/metabolism Species Specificity Viral Proteins/metabolism
Chemicals
Codon Peptide Initiation Factors RNA, Ribosomal RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bassel B A
Mills D R
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.5.2003
Published
1979-00-00
Pages
2003-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327827
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.5.2003 PMC Full Text View journal details
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