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PMID: 819656 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli: some modifications in the Sanger method for RNA sequencing.

Journal of molecular evolution ·Vol. 7 ·No. 3 ·1976-04-09 ·Pages 197-213

Woese C, Sogin M, Stahl D, Lewis BJ, Bonen L

Abstract

Two modifications in the Sanger two dimensional electrophoretic procedure for RNA analysis are reported. One increases resolution on the primary fingerprint to the point that digests of large RNAs, of the size 1500-3000 nucleotides yield well resolved fingerprint patterns. The other is a novel endonucleolytic procedure that proves useful in determining sequences of the large oligonucleotides produced by T1 ribonuclease. These modifications have been used in determining the catalogs of oligomers produced by T1 ribonuclease digestion of 16S rRNAs from three related organisms, Bacillus subtilis, B.pumilus and B.stearothermophilus. The possible effects of adaptation to a thermophilic niche on ribosomal RNA primary structure and the phylogenetic relatedness of the two mesophilic Bacilli are discussed.

MeSH Terms

Animals Bacillus Bacillus subtilis Base Sequence Biological Evolution Dogs Geobacillus stearothermophilus Humans Infant, Newborn Phylogeny RNA, Bacterial/analysis RNA, Ribosomal/analysis Species Specificity

Chemicals

RNA, Bacterial RNA, Ribosomal

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Woese C

Sogin M

Stahl D

Lewis B J

Bonen L

References (13)

13 references, click to expand

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Article Info

Journal

Journal of molecular evolution

Abbr.

J Mol Evol

ISSN

0022-2844

DOI

10.1007/BF01731489

Published

1976-04-09

Pages

197-213

Language

English

Region

Germany

NLM ID

0360051

Subset

External Links

PubMed source DOI: 10.1007/BF01731489 View journal details

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A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli: some modifications in the Sanger method for RNA sequencing.

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