Home LiteratureArticle Details
PMID: 10931913 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

Analysis of the splicing machinery in fission yeast: a comparison with budding yeast and mammals.

Nucleic acids research ·Vol. 28 ·No. 16 ·2000-08-15 ·Pages 3003-10

Käufer NF, Potashkin J

Abstract

Based on genetic and bioinformatic analysis, 80 proteins from the newly sequenced Schizosaccharomyces pombe genome appear to be splicing factors. The fission yeast splicing factors were compared to those of Homo sapiens and Saccharomyces cerevisiae in order to determine the extent of conservation or divergence that has occurred over the billion years of evolution that separate these organisms. Our results indicate that many of the factors present in all three organisms have been well conserved throughout evolution. It is calculated that 38% of the fission yeast splicing factors are more similar to the human proteins than to the budding yeast proteins (>10% more similar or similar over a greater region). Many of the factors in this category are required for recognition of the 3' splice site. Ten fission yeast splicing factors, including putative regulatory factors, have human homologs, but no apparent budding yeast homologs based on sequence data alone. Many of the budding yeast factors that are absent in fission yeast are associated with the U1 and U4/U6.U5 snRNP. Collectively the data presented in this survey indicate that of the two yeasts, S.POMBE: contains a splicing machinery more closely reflecting the archetype of a spliceosome.

MeSH Terms
Animals Fungal Proteins/genetics Genetic Variation Genome Humans Mammals RNA Splicing/genetics Saccharomyces cerevisiae/genetics Schizosaccharomyces/genetics
Chemicals
Fungal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Käufer N F
Institut für Genetik-Biozentrum, Technische Universität Braunschweig, 38106 Braunschweig, Germany.
Potashkin J
References (40)
40 references, click to expand
  1. The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8742-6 PMID: 2479028
  2. Pre-mRNA splicing mutants of Schizosaccharomyces pombe.
    EMBO J. 1989 Feb;8(2):551-9 PMID: 2721492
  3. Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein.
    Nature. 1989 Dec 14;342(6251):819-21 PMID: 2532307
  4. Architectural features of pre-mRNA introns in the fission yeast Schizosaccharomyces pombe.
    Yeast. 1992 Mar;8(3):171-82 PMID: 1574925
  5. A mitotic role for a novel fission yeast protein kinase dsk1 with cell cycle stage dependent phosphorylation and localization.
    Mol Biol Cell. 1993 Mar;4(3):247-60 PMID: 8485317
  6. The fission yeast prp4+ gene involved in pre-mRNA splicing codes for a predicted serine/threonine kinase and is essential for growth.
    Nucleic Acids Res. 1993 Aug 25;21(17):4079-83 PMID: 8371982
  7. U2AF homolog required for splicing in vivo.
    Science. 1993 Oct 22;262(5133):573-5 PMID: 8211184
  8. The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition.
    Genes Dev. 1994 Apr 1;8(7):843-54 PMID: 7926772
  9. The small subunit of the splicing factor U2AF is conserved in fission yeast.
    Nucleic Acids Res. 1996 May 15;24(10):1849-54 PMID: 8657565
  10. SR proteins and splicing control.
    Genes Dev. 1996 Jul 1;10(13):1569-79 PMID: 8682289
  11. Initial splice-site recognition and pairing during pre-mRNA splicing.
    Curr Opin Genet Dev. 1996 Apr;6(2):215-20 PMID: 8722179
  12. Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat.
    Science. 1996 Oct 25;274(5287):605-10 PMID: 8849451
  13. A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase.
    Mol Biol Cell. 1996 Jul;7(7):1083-94 PMID: 8862522
  14. Allelism of PSO4 and PRP19 links pre-mRNA processing with recombination and error-prone DNA repair in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1996 Oct 15;24(20):4009-14 PMID: 8918805
  15. Functional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologue.
    Nucleic Acids Res. 1997 Mar 1;25(5):1028-35 PMID: 9102632
  16. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals.
    Cell. 1997 May 2;89(3):403-12 PMID: 9150140
  17. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
    Cell. 1997 May 30;89(5):781-7 PMID: 9182766
  18. The prp1+ gene required for pre-mRNA splicing in Schizosaccharomyces pombe encodes a protein that contains TPR motifs and is similar to Prp6p of budding yeast.
    Genetics. 1997 Sep;147(1):101-15 PMID: 9286671
  19. Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.
    Curr Genet. 1997 Nov;32(5):323-30 PMID: 9371883
  20. Fission yeast mitotic regulator Dsk1 is an SR protein-specific kinase.
    J Biol Chem. 1998 Mar 6;273(10):5963-9 PMID: 9488736
  21. A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.
    Mol Cell Biol. 1998 Aug;18(8):4752-60 PMID: 9671485
  22. CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif.
    Mol Cell Biol. 1998 Sep;18(9):5000-9 PMID: 9710584
  23. Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.
    Nat Genet. 1998 Sep;20(1):46-50 PMID: 9731529
  24. The fission yeast prp10(+) gene involved in pre-mRNA splicing encodes a homologue of highly conserved splicing factor, SAP155.
    Nucleic Acids Res. 1998 Dec 15;26(24):5662-9 PMID: 9837997
  25. The type 2C Ser/Thr phosphatase PP2Cgamma is a pre-mRNA splicing factor.
    Genes Dev. 1999 Jan 1;13(1):87-97 PMID: 9887102
  26. An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p.
    Mol Cell. 1999 Jan;3(1):55-64 PMID: 10024879
  27. Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing.
    Genes Dev. 1999 Apr 1;13(7):841-50 PMID: 10197984
  28. Srp2, an SR protein family member of fission yeast: in vivo characterization of its modular domains.
    Nucleic Acids Res. 1999 Jul 1;27(13):2618-26 PMID: 10373577
  29. Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing.
    Mol Cell Biol. 1999 Aug;19(8):5352-62 PMID: 10409726
  30. Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.
    Genes Dev. 1999 Aug 1;13(15):1970-82 PMID: 10444595
  31. The best yeast?
    Trends Genet. 1999 Sep;15(9):340-4 PMID: 10461200
  32. spp42, identified as a classical suppressor of prp4-73, which encodes a kinase involved in pre-mRNA splicing in fission yeast, is a homologue of the splicing factor Prp8p.
    Genetics. 1999 Nov;153(3):1183-91 PMID: 10545451
  33. Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing.
    Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13789-94 PMID: 10570151
  34. Functional recognition of the 3' splice site AG by the splicing factor U2AF35.
    Nature. 1999 Dec 16;402(6763):832-5 PMID: 10617206
  35. Both subunits of U2AF recognize the 3' splice site in Caenorhabditis elegans.
    Nature. 1999 Dec 16;402(6763):835-8 PMID: 10617207
  36. Inhibition of msl-2 splicing by Sex-lethal reveals interaction between U2AF35 and the 3' splice site AG.
    Nature. 1999 Dec 16;402(6763):838-41 PMID: 10617208
  37. Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1.
    Mol Cell Biol. 2000 Feb;20(3):816-24 PMID: 10629038
  38. The RNA splicing factor hSlu7 is required for correct 3' splice-site choice.
    Nature. 1999 Nov 11;402(6758):207-10 PMID: 10647016
  39. Fission yeast Schizosaccharomyces pombe correctly excises a mammalian RNA transcript intervening sequence.
    Nature. 1985 Nov 7-13;318(6041):78-80 PMID: 2997624
  40. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9243-7 PMID: 2531895
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/28.16.3003
Published
2000-08-15
Pages
3003-10
Language
English
Region
England
NLM ID
0411011
PMCID
PMC108416
Subset
IM
Grants
NIGMS NIH HHS · R01GM47487 · United States
External Links
PubMed source DOI: 10.1093/nar/28.16.3003 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2