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PMID: 10906222 Published · ppublish English Journal Article

Complete DNA sequence of the rat cytomegalovirus genome.

Journal of virology ·Vol. 74 ·No. 16 ·2000-08-00 ·Pages 7656-65

Vink C, Beuken E, Bruggeman CA

Abstract

We have determined the complete genome sequence of the Maastricht strain of rat cytomegalovirus (RCMV). The RCMV genome has a length of 229,896 bp and is arranged as a single unique sequence flanked by 504-bp terminal direct repeats. RCMV was found to have counterparts of all but one of the open reading frames (ORFs) that are conserved between murine CMV (MCMV) and human CMV (HCMV). Like HCMV, RCMV lacks homologs of the genes belonging to the MCMV m02 glycoprotein gene family. However, RCMV contains 15 ORFs with homology to members of the MCMV m145 glycoprotein gene family. Four ORFs are predicted to encode homologs of host proteins; R33 and R78 both putatively encode G protein-coupled receptors, whereas r144 and r131 encode homologs of major histocompatibility class I heavy chains and CC chemokines, respectively. An intriguing feature of the RCMV genome is the presence of an ORF, r127, with similarity to the rep gene of parvoviruses as well as ORF U94 of human herpesvirus 6A (HHV-6A) and HHV-6B. Counterparts of these ORFs have not been found in the other sequenced herpesviruses.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Replication/genetics DNA, Viral/metabolism Genome, Viral Humans Mice Molecular Sequence Data Muromegalovirus/genetics Open Reading Frames/genetics RNA Splicing Rats Sequence Alignment Sequence Analysis, DNA Viral Proteins/chemistry,genetics
Chemicals
DNA, Viral Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vink C
Department of Medical Microbiology, Cardiovascular Research Institute Maastricht, University of Maastricht, 6202 AZ Maastricht, The Netherlands. kvi@lmib.azm.nl
Beuken E
Bruggeman C A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.74.16.7656-7665.2000
Email
kvi@lmib.azm.nl
Published
2000-08-00
Pages
7656-65
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112289
Subset
IM
Databases
GENBANK
AF232689
External Links
PubMed source DOI: 10.1128/jvi.74.16.7656-7665.2000 PMC Full Text View journal details
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