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PMID: 9445038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Virus attenuation after deletion of the cytomegalovirus Fc receptor gene is not due to antibody control.

Journal of virology ·Vol. 72 ·No. 2 ·1998-02-00 ·Pages 1377-82

Crnković-Mertens I, Messerle M, Milotić I, Szepan U, Kucić N, Krmpotić A, Jonjić S, Koszinowski UH

Abstract

The murine cytomegalovirus (MCMV) fcr-1 gene codes for a glycoprotein located at the surface of infected cells which strongly binds the Fc fragment of murine immunoglobulin G. To determine the biological significance of the fcr-1 gene during viral infection, we constructed MCMV fcr-1 deletion mutants and revertants. The fcr-1 gene was disrupted by insertion of the Escherichia coli lacZ gene. In another mutant, the marker gene was also deleted, by recombinase cre. As expected for its hypothetical role in immunoevasion, the infection of mice with fcr-1 deletion mutants resulted in significantly restricted replication in comparison with wild-type MCMV and revertant virus. In mutant mice lacking antibodies, however, the fcr-1 deletion mutants also replicated poorly. This demonstrated that the cell surface-expressed viral glycoprotein with FcR activity strongly modulates the virus-host interaction but that this biological function is not caused by the immunoglobulin binding property.

MeSH Terms
3T3 Cells Animals Antibodies, Viral/immunology Gene Deletion Glycoproteins/immunology Immunoglobulin Fc Fragments/immunology Immunoglobulin G/immunology Membrane Glycoproteins/immunology Mice Muromegalovirus/genetics,immunology Receptors, Fc/genetics,immunology Viral Proteins
Chemicals
Antibodies, Viral Fcr-1 protein, Mouse cytomegalovirus 1 Glycoproteins Immunoglobulin Fc Fragments Immunoglobulin G Membrane Glycoproteins Receptors, Fc Viral Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Crnković-Mertens I
Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, University of Munich, Germany.
Messerle M
Milotić I
Szepan U
Kucić N
Krmpotić A
Jonjić S
Koszinowski U H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.2.1377-1382.1998
Published
1998-02-00
Pages
1377-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC124616
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.72.2.1377-1382.1998 PMC Full Text View journal details
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