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PMID: 1090576 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.

Journal of bacteriology ·Vol. 121 ·No. 1 ·1975-01-00 ·Pages 381-9

Inselburg J, Johns V

Abstract

Colicin plasmids E2 and E3 (Col E2 and Col E3) deoxyribonucleic acid (DNA) has been shown to contain, respectively, two and three EcoR1 restriction endonuclease-sensitive sites. This was determined by measuring the DNA fragments generated after EcoR1 endonuclease treatment by agarose gel electrophoresis and electron microscopy. The structure of heteroduplex Col E2-col E3 DNA molecules formed from EcoR1-generated fragments permitted a localization of the EcoR1-sensitive sites on the plasmid chromosomes.

MeSH Terms

Binding Sites Colicins/biosynthesis DNA, Bacterial/metabolism DNA, Circular/metabolism Electrophoresis Endonucleases/metabolism Escherichia coli/metabolism Extrachromosomal Inheritance Formamides Microscopy, Electron Molecular Weight Nucleic Acid Conformation Nucleic Acid Denaturation

Chemicals

Colicins DNA, Bacterial DNA, Circular Formamides Endonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Inselburg J

Johns V

References (13)

13 references, click to expand

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A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
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[Preparation and length measurements of the total desoxyribonucleic acid content of T2 bacteriophages].
Biochim Biophys Acta. 1962 Dec 31;61:857-64 PMID: 14033431
Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4 PMID: 4601821
Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3370-4 PMID: 4343968
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.121.1.381-389.1975

Published

1975-01-00

Pages

381-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285653

Subset

External Links

PubMed source DOI: 10.1128/jb.121.1.381-389.1975 PMC Full Text View journal details

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Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.

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