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PMID: 10889249 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tissue-preferential expression of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron.

Plant physiology ·Vol. 123 ·No. 3 ·2000-07-00 ·Pages 1005-14

Jeon JS, Lee S, Jung KH, Jun SH, Kim C, An G

Abstract

The genomic clone encoding an alpha-tubulin, OsTubA1, has been isolated from rice (Oryza sativa L.). The gene consists of four exons and three introns. RNA-blot analysis showed that the gene is strongly expressed in actively dividing tissues, including root tips, young leaves, and young flowers. Analysis of chimeric fusions between OsTubA1 and beta-glucuronidase (GUS) revealed that the intron 1 was required for high-level GUS expression in actively dividing tissues, corresponding with normal expression pattern of OsTubA1. Fusion constructs lacking the intron 1 showed more GUS staining in mature tissues rather than young tissues. When the intron 1 was placed at the distal region from 5'-upstream region or at the 3'-untranslated region, no enhancement of GUS expression was observed. Sequential deletions of the OsTubA1 intron 1 brought about a gradual reduction of GUS activity in calli. These results suggest that tissue-preferential expression of the OsTubA1 gene is mediated by the intron 1 and that it may be involved in a mechanism for an efficient RNA splicing that is position dependent.

MeSH Terms
Blotting, Northern Gene Expression Regulation, Plant Genes, Reporter Glucuronidase/genetics,metabolism Introns Oryza/genetics,metabolism Plants, Genetically Modified RNA, Messenger/analysis Recombinant Fusion Proteins/genetics,metabolism Tubulin/genetics,metabolism
Chemicals
RNA, Messenger Recombinant Fusion Proteins Tubulin Glucuronidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jeon J S
Department of Life Science and National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Lee S
Jung K H
Jun S H
Kim C
An G
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.123.3.1005
Published
2000-07-00
Pages
1005-14
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC59063
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.123.3.1005 PMC Full Text View journal details
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