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PMID: 10888664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Exo1 roles for repair of DNA double-strand breaks and meiotic crossing over in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 11 ·No. 7 ·2000-07-00 ·Pages 2221-33

Tsubouchi H, Ogawa H

Abstract

The MRE11, RAD50, and XRS2 genes of Saccharomyces cerevisiae are involved in the repair of DNA double-strand breaks (DSBs) produced by ionizing radiation and by radiomimetic chemicals such as methyl methanesulfonate (MMS). In these mutants, single-strand DNA degradation in a 5' to 3' direction from DSB ends is reduced. Multiple copies of the EXO1 gene, encoding a 5' to 3' double-strand DNA exonuclease, were found to suppress the high MMS sensitivity of these mutants. The exo1 single mutant shows weak MMS sensitivity. When an exo1 mutation is combined with an mre11 mutation, both repair of MMS-induced damage and processing of DSBs are more severely reduced than in either single mutant, suggesting that Exo1 and Mre11 function independently in DSB processing. During meiosis, transcription of the EXO1 gene is highly induced. In meiotic cells, the exo1 mutation reduces the processing of DSBs and the frequency of crossing over, but not the frequency of gene conversion. These results suggest that Exo1 functions in the processing of DSB ends and in meiotic crossing over.

MeSH Terms
Crossing Over, Genetic DNA Damage DNA Repair DNA, Fungal Endodeoxyribonucleases Exodeoxyribonucleases/genetics,physiology Fungal Proteins/genetics,physiology Genes, Fungal Meiosis/physiology Methyl Methanesulfonate Phenotype Plasmids Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Spores, Fungal Telomere Transcription, Genetic
Chemicals
DNA, Fungal Fungal Proteins Saccharomyces cerevisiae Proteins Methyl Methanesulfonate Endodeoxyribonucleases Exodeoxyribonucleases MRE11 protein, S cerevisiae exodeoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tsubouchi H
Department of Biology, Graduate School of Science, Osaka University, Osaka 560-0043, Japan.
Ogawa H
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.7.2221
Published
2000-07-00
Pages
2221-33
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC14915
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.11.7.2221 PMC Full Text View journal details
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