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PMID: 10848619 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.

Molecular and cellular biology ·Vol. 20 ·No. 13 ·2000-07-00 ·Pages 4948-57

Ward TR, Hoang ML, Prusty R, Lau CK, Keil RL, Fangman WL, Brewer BJ

Abstract

In the ribosomal DNA of Saccharomyces cerevisiae, sequences in the nontranscribed spacer 3' of the 35S ribosomal RNA gene are important to the polar arrest of replication forks at a site called the replication fork barrier (RFB) and also to the cis-acting, mitotic hyperrecombination site called HOT1. We have found that the RFB and HOT1 activity share some but not all of their essential sequences. Many of the mutations that reduce HOT1 recombination also decrease or eliminate fork arrest at one of two closely spaced RFB sites, RFB1 and RFB2. A simple model for the juxtaposition of RFB and HOT1 sequences is that the breakage of strands in replication forks arrested at RFB stimulates recombination. Contrary to this model, we show here that HOT1-stimulated recombination does not require the arrest of forks at the RFB. Therefore, while HOT1 activity is independent of replication fork arrest, HOT1 and RFB require some common sequences, suggesting the existence of a common trans-acting factor(s).

MeSH Terms
DNA Replication DNA, Fungal/chemistry,genetics,metabolism DNA, Ribosomal/chemistry,genetics,metabolism Deoxyribonuclease HindIII/genetics,metabolism Deoxyribonucleases, Type II Site-Specific/genetics,metabolism Mutation Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/genetics
Chemicals
DNA, Fungal DNA, Ribosomal HOT1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Deoxyribonuclease HindIII endodeoxyribonuclease HpaI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ward T R
Department of Genetics, University of Washington, Seattle 98195, USA.
Hoang M L
Prusty R
Lau C K
Keil R L
Fangman W L
Brewer B J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.13.4948-4957.2000
Published
2000-07-00
Pages
4948-57
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85945
Subset
IM
Grants
NIGMS NIH HHS · T32 GM007735 · United States
NIGMS NIH HHS · R01 GM36422 · United States
NIGMS NIH HHS · T32 GM07735 · United States
External Links
PubMed source DOI: 10.1128/MCB.20.13.4948-4957.2000 PMC Full Text View journal details
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