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PMID: 10722590 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutations in the extracellular protein secretion pathway genes (eps) interfere with rugose polysaccharide production in and motility of Vibrio cholerae.

Infection and immunity ·Vol. 68 ·No. 4 ·2000-04-00 ·Pages 1967-74

Ali A, Johnson JA, Franco AA, Metzger DJ, Connell TD, Morris JG, Sozhamannan S

Abstract

Vibrio cholerae is the causal organism of the diarrheal disease cholera. The rugose variant of V. cholerae is associated with the secretion of an exopolysaccharide. The rugose polysaccharide has been shown to confer increased resistance to a variety of agents, such as chlorine, bioacids, and oxidative and osmotic stresses. It also promotes biofilm formation, thereby increasing the survival of the bacteria in the aquatic environments. Here we show that the extracellular protein secretion system (gene designated eps) is involved directly or indirectly in the production of rugose polysaccharide. A TnphoA insertion in epsD gene of the eps operon abolished the production of rugose polysaccharide, reduced the secretion of cholera toxin and hemolysin, and resulted in a nonmotile phenotype. We have constructed defined mutations of the epsD and epsE genes that affected these phenotypes and complemented these defects by plasmid clones of the respective wild-type genes. These results suggest a major role for the eps system in pathogenesis and environmental survival of V. cholerae.

MeSH Terms
Alkaline Phosphatase Bacterial Proteins/genetics,physiology Cholera Toxin/biosynthesis Cloning, Molecular Cosmids Cyclin-Dependent Kinases/genetics Flagella/physiology Genetic Complementation Test Hemolysin Proteins/biosynthesis Membrane Proteins Movement Mutagenesis Mutation Operon Phenotype Polysaccharides/biosynthesis Vibrio cholerae/genetics,metabolism,physiology
Chemicals
Bacterial Proteins EpsD protein, Vibrio cholerae EpsE protein, Vibrio cholerae Hemolysin Proteins Membrane Proteins Polysaccharides Cholera Toxin Cyclin-Dependent Kinases Alkaline Phosphatase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ali A
Division of Hospital Epidemiology, Department of Medicine, University of Maryland at Baltimore, Maryland 21201, USA.
Johnson J A
Franco A A
Metzger D J
Connell T D
Morris J G
Sozhamannan S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
2000-04-00
Pages
1967-74
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97374
Subset
IM
Grants
NIAID NIH HHS · AI 35729 · United States
Corrections
ErratumIn
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