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PMID: 10712515 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of two RNA-binding proteins associated with human telomerase RNA.

Molecular biology of the cell ·Vol. 11 ·No. 3 ·2000-03-00 ·Pages 999-1010

Le S, Sternglanz R, Greider CW

Abstract

Telomerase plays a crucial role in telomere maintenance in vivo. To understand telomerase regulation, we have been characterizing components of the enzyme. To date several components of the mammalian telomerase holoenzyme have been identified: the essential RNA component (human telomerase RNA [hTR]), the catalytic subunit human telomerase reverse transcriptase (hTERT), and telomerase-associated protein 1. Here we describe the identification of two new proteins that interact with hTR: hStau and L22. Antisera against both proteins immunoprecipitated hTR, hTERT, and telomerase activity from cell extracts, suggesting that the proteins are associated with telomerase. Both proteins localized to the nucleolus and cytoplasm. Although these proteins are associated with telomerase, we found no evidence of their association with each other or with telomerase-associated protein 1. Both hStau and L22 are more abundant than TERT. This, together with their localization, suggests that they may be associated with other ribonucleoprotein complexes in cells. We propose that these two hTR-associated proteins may play a role in hTR processing, telomerase assembly, or localization in vivo.

MeSH Terms
Amino Acid Sequence Biological Transport Cloning, Molecular Cytoskeletal Proteins HeLa Cells Humans Jurkat Cells Macromolecular Substances Molecular Sequence Data RNA/metabolism RNA-Binding Proteins/metabolism Ribonucleoproteins/metabolism Ribosomal Proteins Sequence Homology, Amino Acid Subcellular Fractions Telomerase/metabolism
Chemicals
Cytoskeletal Proteins Macromolecular Substances RNA-Binding Proteins Ribonucleoproteins Ribosomal Proteins STAU1 protein, human staufen protein, mammalian RPL22 protein, human RNA Telomerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Le S
Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Sternglanz R
Greider C W
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.3.999
Published
2000-03-00
Pages
999-1010
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC14826
Subset
IM
Grants
NCI NIH HHS · F32 CA068736 · United States
NIGMS NIH HHS · R56 GM028220 · United States
NIGMS NIH HHS · GM-28220 · United States
NIGMS NIH HHS · R01 GM028220 · United States
NCI NIH HHS · CA-68736 · United States
NIA NIH HHS · R37 AG009383 · United States
NIA NIH HHS · AG-09383 · United States
External Links
PubMed source DOI: 10.1091/mbc.11.3.999 PMC Full Text View journal details
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