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PMID: 1069999 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Modification of adenylate cyclase activity in LM cells by manipulation of the membrane phospholipid composition in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 12 ·1976-12-00 ·Pages 4482-6

Engelhard VH, Esko JD, Storm DR, Glaser M

Abstract

Adenylate cyclase [atp pyrophosphate-lyase (cyclizing): EC 4.6.4.4] activities were examined in mouse LM cell (fibroblast) membranes that were supplemented with ethanolamine and/or fatty acids. The supplements were incorporated into the plasma membrane phospholipids in significant amounts. Fatty acid supplementations had distinct effects as compared to polar head group supplementations. All lipid supplementations increased basal adenylate cyclase activity relative to control cells grown in choline-containing medium. Double supplementation with ethanolamine and linoleate increased the specific activity of adenylate cyclase up to 4-fold. Activity in the presence of fluoride was unaffected by ethanolamine supplementation, but was increased by fatty acid supplementation. In contrast, prostaglandin E1 stimulation was 4.2-fold in controls and ethanolamine and/or elaidate supplements, 6-fold in choline plus linoleate supplements, and 3.1-fold in ethanolamine plus linoleate supplements. Differences in activity could not be ascribed to changes in membrane protein composition in supplemented cells, and could be abolished by detergent solublization. Fluidity of the supplemented membranes was monitored by fluorescence polarization, and no correlation was observed between membrane viscosity and adenylate cyclase activity or hormone stimulation. These results emphasize the importance of the membrane lipid phase for this enzyme.

MeSH Terms
Adenylyl Cyclases/metabolism Cell Line Choline Detergents Enzyme Activation Ethanolamines Fatty Acids Fluorides/pharmacology Membrane Lipids/physiology Phospholipids/physiology Prostaglandins E/pharmacology Solubility Structure-Activity Relationship Temperature Viscosity
Chemicals
Detergents Ethanolamines Fatty Acids Membrane Lipids Phospholipids Prostaglandins E Adenylyl Cyclases Choline Fluorides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Engelhard V H
Esko J D
Storm D R
Glaser M
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.12.4482
Published
1976-12-00
Pages
4482-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431510
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.12.4482 PMC Full Text View journal details
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