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PMID: 10699017 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular characterization of Cryptosporidium isolates obtained from human immunodeficiency virus-infected individuals living in Switzerland, Kenya, and the United States.

Journal of clinical microbiology ·Vol. 38 ·No. 3 ·2000-03-00 ·Pages 1180-3

Morgan U, Weber R, Xiao L, Sulaiman I, Thompson RC, Ndiritu W, Lal A, Moore A, Deplazes P

Abstract

A total of 22 Cryptosporidium isolates from human immunodeficiency virus-infected patients from Kenya, Switzerland, and the United States were examined at three genetic loci: the 18S ribosomal DNA, HSP-70, and acetyl coenzyme A synthetase genes. Four distinct Cryptosporidium genotypes were identified: (i) the Cryptosporidium parvum "human" genotype, (ii) the C. parvum "cattle" genotype, (iii) Cryptosporidium felis, and (iv) Cryptosporidium meleagridis. This is the first report of C. meleagridis in a human host. These results and those of others indicate that immunocompromised individuals are susceptible to a wide range of Cryptosporidium species and genotypes. Future studies are required to understand the full public health significance of Cryptosporidium genotypes and species in immunocompromised hosts.

MeSH Terms
AIDS-Related Opportunistic Infections/diagnosis,parasitology Acetate-CoA Ligase/genetics Animals Cryptosporidiosis/diagnosis Cryptosporidium/classification,genetics,isolation & purification DNA, Protozoan/genetics DNA, Ribosomal/genetics HIV Infections/complications,transmission HSP70 Heat-Shock Proteins/genetics Homosexuality, Male Humans Kenya Male RNA, Protozoan/genetics RNA, Ribosomal, 18S/genetics Substance Abuse, Intravenous Switzerland United States
Chemicals
DNA, Protozoan DNA, Ribosomal HSP70 Heat-Shock Proteins RNA, Protozoan RNA, Ribosomal, 18S Acetate-CoA Ligase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Morgan U
World Health Organisation Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and State Agricultural Biotechnology Centre, Murdoch University, Murdoch, WA 6150, Australia. morgan@numbat.murdoch.edu.au
Weber R
Xiao L
Sulaiman I
Thompson R C
Ndiritu W
Lal A
Moore A
Deplazes P
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16 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.38.3.1180-1183.2000
Email
morgan@numbat.murdoch.edu.au
Published
2000-03-00
Pages
1180-3
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC86369
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.38.3.1180-1183.2000 PMC Full Text View journal details
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