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PMID: 10698942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dishevelled phosphorylation, subcellular localization and multimerization regulate its role in early embryogenesis.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 1010-22

Rothbächer U, Laurent MN, Deardorff MA, Klein PS, Cho KW, Fraser SE

Abstract

Dishevelled (Dsh) induces a secondary axis and can translocate to the membrane when activated by Frizzleds; however, dominant-negative approaches have not supported a role for Dsh in primary axis formation. We demonstrate that the Dsh protein is post-translationally modified at the dorsal side of the embryo: timing and position of this regulation suggests a role of Dsh in dorsal-ventral patterning in Xenopus. To create functional links between these properties of Dsh we analyzed the influence of endogenous Frizzleds and the Dsh domain dependency for these characteristics. Xenopus Frizzleds phosphorylate and translocate Xdsh to the membrane irrespective of their differential ectopic axes inducing abilities, showing that translocation is insufficient for axis induction. Dsh deletion analysis revealed that axis inducing abilities did not segregate with Xdsh membrane association. The DIX region and a short stretch at the N-terminus of the DEP domain are necessary for axis induction while the DEP region is required for Dsh membrane association and its phosphorylation. In addition, Dsh forms homomeric complexes in embryos suggesting that multimerization is important for its proper function.

MeSH Terms
Adaptor Proteins, Signal Transducing Animals Biological Transport Dishevelled Proteins Embryo, Nonmammalian/physiology Gene Expression Regulation, Developmental Insect Proteins/chemistry,physiology Phosphoproteins/chemistry,physiology Phosphorylation Xenopus/embryology,physiology Xenopus Proteins
Chemicals
Adaptor Proteins, Signal Transducing DVL1 protein, Xenopus Dishevelled Proteins Insect Proteins Phosphoproteins Xenopus Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rothbächer U
Division of Biology and Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA. rothbach@lgpd.univ-mrs.fr
Laurent M N
Deardorff M A
Klein P S
Cho K W
Fraser S E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.1010
Email
rothbach@lgpd.univ-mrs.fr
Published
2000-03-01
Pages
1010-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305640
Subset
IM
Grants
NICHD NIH HHS · R01 HD029507 · United States
NICHD NIH HHS · HD25390 · United States
NICHD NIH HHS · HD29507 · United States
NIMH NIH HHS · MH49176 · United States
External Links
PubMed source DOI: 10.1093/emboj/19.5.1010 PMC Full Text View journal details
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