Home LiteratureArticle Details

PMID: 10073940 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin.

Science (New York, N.Y.) ·Vol. 283 ·No. 5408 ·1999-03-12 ·Pages 1739-42

Hamada F, Tomoyasu Y, Takatsu Y, Nakamura M, Nagai S, Suzuki A, Fujita F, Shibuya H, Toyoshima K, Ueno N, Akiyama T

Abstract

Wnt/Wingless directs many cell fates during development. Wnt/Wingless signaling increases the amount of beta-catenin/Armadillo, which in turn activates gene transcription. Here the Drosophila protein D-Axin was shown to interact with Armadillo and D-APC. Mutation of d-axin resulted in the accumulation of cytoplasmic Armadillo and one of the Wingless target gene products, Distal-less. Ectopic expression of d-axin inhibited Wingless signaling. Hence, D-Axin negatively regulates Wingless signaling by down-regulating the level of Armadillo. These results establish the importance of the Axin family of proteins in Wnt/Wingless signaling in Drosophila.

MeSH Terms

Adaptor Proteins, Signal Transducing Adenomatous Polyposis Coli Protein Animals Armadillo Domain Proteins Axin Protein Body Patterning Carrier Proteins/chemistry,genetics,metabolism Chromosome Mapping Cytoplasm/metabolism Cytoskeletal Proteins/metabolism Down-Regulation Drosophila/embryology,genetics,metabolism Drosophila Proteins Embryo, Nonmammalian/metabolism Extremities/embryology Gene Expression Regulation, Developmental Genes, Insect Homeodomain Proteins/genetics,metabolism In Situ Hybridization Insect Proteins/genetics,metabolism Molecular Sequence Data Mutation Phenotype Proteins/chemistry,genetics,metabolism Proto-Oncogene Proteins/metabolism Recombinant Fusion Proteins/metabolism Repressor Proteins Signal Transduction Trans-Activators Transcription Factors Wings, Animal/embryology,metabolism Wnt1 Protein

Chemicals

APC protein, Drosophila ARM protein, Drosophila Adaptor Proteins, Signal Transducing Adenomatous Polyposis Coli Protein Armadillo Domain Proteins Axin Protein Axn protein, Drosophila Carrier Proteins Cytoskeletal Proteins Drosophila Proteins Homeodomain Proteins Insect Proteins Proteins Proto-Oncogene Proteins Recombinant Fusion Proteins Repressor Proteins Trans-Activators Transcription Factors Wnt1 Protein distal-less protein, insect wg protein, Drosophila

Authors & Affiliations

11 authors, click to expand affiliations / ORCID

Hamada F

Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, Japan.

Tomoyasu Y

Takatsu Y

Nakamura M

Nagai S

Suzuki A

Fujita F

Shibuya H

Toyoshima K

Ueno N

Akiyama T

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.283.5408.1739

Published

1999-03-12

Pages

1739-42

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

AF086811

External Links

PubMed source DOI: 10.1126/science.283.5408.1739 View journal details

Analysis Services

Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin.

Contact

WeChat Official Account