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PMID: 1069293 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Tubulin requires tau for growth onto microtubule initiating sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 4070-4

Witman GB, Cleveland DW, Weingarten MD, Kirschner MW

Abstract

Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.

MeSH Terms
Animals Flagella Glycoproteins/metabolism Kinetics Microscopy, Electron Microtubules/metabolism Molecular Weight Proteins/metabolism Tubulin/metabolism
Chemicals
Glycoproteins Proteins Tubulin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Witman G B
Cleveland D W
Weingarten M D
Kirschner M W
References (26)
26 references, click to expand
  1. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  2. Directionality of brain microtubule assembly in vitro.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1710-4 PMID: 4134974
  3. Simple method for quantitive densitometry of polyacrylamide gels using fast green.
    Anal Biochem. 1970 Jun;35(2):359-70 PMID: 4194859
  4. Microtubule assembly in the absence of added nucleotides.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8 PMID: 4514990
  5. Comparison of the sedimentation properties of microtubule protein oligomers prepared by two different procedures.
    Biochem Biophys Res Commun. 1976 May 3;70(1):1-7 PMID: 1275930
  6. Microtubule formation in vitro in solutions containing low calcium concentrations.
    Science. 1972 Sep 22;177(4054):1104-5 PMID: 4626639
  7. Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.
    J Cell Biol. 1972 Sep;54(3):507-39 PMID: 4558009
  8. The mechanism of microtubule assembly in vitro.
    J Supramol Struct. 1974;2(2-4):412-28 PMID: 4474574
  9. Assembly of microtubules from preformed, ring-shaped protofilaments and 6-S tubulin.
    J Supramol Struct. 1974;2(2-4):393-411 PMID: 4474573
  10. Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules.
    J Mol Biol. 1974 Nov 15;89(4):737-55 PMID: 4475698
  11. Structural polarity and directional growth of microtubules of Chlamydomonas flagella.
    J Mol Biol. 1974 Dec 5;90(2):381-402 PMID: 4476803
  12. Interpretation of the light scattering from long rods.
    J Mol Biol. 1974 Nov 15;89(4):755-8 PMID: 4449130
  13. Properties of the depolymerization products of microtubules from mammalian brain.
    Biochemistry. 1974 Dec 31;13(27):5529-37 PMID: 4457112
  14. Light-microscopic observations of individual microtubules reconstituted from brain tubulin.
    J Cell Sci. 1975 Dec;19(3):607-20 PMID: 173726
  15. Nucleated assembly of microtubules in porcine brain extracts.
    Science. 1972 Sep 29;177(4055):1196-7 PMID: 5057625
  16. The reconstitution of microtubules from purified calf brain tubulin.
    Biochemistry. 1975 Nov 18;14(23):5183-7 PMID: 811253
  17. Microtubule formation from two components separated by gel filtration of a tubulin preparation.
    Biochim Biophys Acta. 1975 Jun 12;392(2):335-45 PMID: 805601
  18. The site of in vivo assembly of flagellar microtubules.
    Ann N Y Acad Sci. 1975 Jun 30;253:178-91 PMID: 1056740
  19. Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2696-700 PMID: 1058484
  20. A protein factor essential for microtubule assembly.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1858-62 PMID: 1057175
  21. Assembly of chick brain tubulin onto flagellar microtubules from Chlamydomonas and sea urchin sperm.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1122-6 PMID: 1055370
  22. Duplex microtubule is a new form of tubulin assembly induced by polycations.
    Nature. 1975 Oct 23;257(5528):707-9 PMID: 1186848
  23. Tubulin requires an accessory protein for self assembly in microtubules.
    Nature. 1975 Oct 2;257(5525):418-21 PMID: 1178045
  24. Quantitative electron microscopy of microtubule assembly in vitro.
    J Mol Biol. 1975 Dec 5;99(2):263-76 PMID: 1206704
  25. Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules.
    J Cell Biol. 1975 Apr;65(1):237-41 PMID: 1127013
  26. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.11.4070
Published
1976-11-00
Pages
4070-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431332
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.11.4070 PMC Full Text View journal details
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