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PMID: 1069261 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation, primary structure, and synthesis of alpha-endorphin and gamma-endorphin, two peptides of hypothalamic-hypophysial origin with morphinomimetic activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 3942-6

Ling N, Burgus R, Guillemin R

Abstract

The isolation and primary structure of two peptides with morphinomimetic activity, obtained from an extract of porcine hypothalamus-neurohypophysis, are described. The amino acid sequence of the two peptides, named alpha-endorphin and gamma-endophin, was determined by mass spectrometry and danxyl-Edman methods to be H-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-OH and H-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-OH, respectively. These correspond to the amino acid sequences present between residues 61 and 76 and residues 61 and 77 of the various beta-lipotropins. A third peptide also obtained in pure form in these studies was found to be an unstable salt of alpha-endorphin.

MeSH Terms
Amino Acid Sequence Animals Hypothalamus/chemistry Nerve Tissue Proteins/analysis,chemical synthesis Peptides/analysis,chemical synthesis Pituitary Gland, Posterior/chemistry Swine
Chemicals
Nerve Tissue Proteins Peptides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ling N
Burgus R
Guillemin R
References (19)
19 references, click to expand
  1. Synthesis of a pentekontapeptide with high lipolytic activity corresponding to the carboxyl-terminal fifty amino acids of ovine beta-lipotropin.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4945-9 PMID: 4531028
  2. [Endorphins, hypothalamic and neurohypophysial peptides with morphinomimetic activity: isolation and molecular structure of alpha-endorphin].
    C R Acad Sci Hebd Seances Acad Sci D. 1976 Feb 23;282(8):783-5 PMID: 817843
  3. Analogs of luteinizing hormone releasing factor (LRF). Synthesis and biological activity of ((Nalpha-Me)Leu-7)LRF and (D-Ala-6,(Nalpha-Me)Leu-7)LRF.
    Biochem Biophys Res Commun. 1975 Apr 7;63(3):801-6 PMID: 1093545
  4. Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145-8 PMID: 1063395
  5. beta-Lipotropin as a prohormone for the morphinomimetic peptides endorphins and enkephalins.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):2156-9 PMID: 1064883
  6. Opioid activity of a peptide, beta-lipotropin-(61-91), derived from beta-lipotropin.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1821-3 PMID: 1064855
  7. Analogues of luteinizing hormone releasing factor modified at positions 2, 6, and 10.
    J Med Chem. 1976 Jul;19(7):937-41 PMID: 781247
  8. C fragment of lipotropin has a high affinity for brain opiate receptors.
    Nature. 1976 Apr 29;260(5554):793-5 PMID: 1264258
  9. Synthesis of a nonadecapeptide corresponding to residues 37-55 of ovine prolactin. 1 Detection and isolation of the sulfonium form of methionine-containing peptides.
    J Am Chem Soc. 1976 Apr 14;98(8):2324-8 PMID: 943436
  10. Identification of two related pentapeptides from the brain with potent opiate agonist activity.
    Nature. 1975 Dec 18;258(5536):577-80 PMID: 1207728
  11. A peptide-like substance from pituitary that acts like morphine. 2. Purification and properties.
    Life Sci. 1975 Jun 15;16(12):1777-82 PMID: 1152601
  12. An endogenous morphine-like factor in mammalian brain.
    Life Sci. 1975 Jun 15;16(12):1765-9 PMID: 1152600
  13. Purification and properties of enkephalin - the possible endogenous ligand for the morphine receptor.
    Life Sci. 1975 Jun 15;16(12):1753-8 PMID: 1152599
  14. [Mass-spectrometric studies, XIV. Effect of aromatic amino acids on electron impulse fragmentation of N-acetyl peptides].
    Justus Liebigs Ann Chem. 1966 Oct;698:24-33 PMID: 5977097
  15. Morphinomimetic activity of synthetic fragments of beta-lipotropin and analogs.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3308-10 PMID: 1067621
  16. The origin of acetylcholine released from guinea-pig intestine and longitudinal muscle strips.
    J Physiol. 1968 Jan;194(1):13-33 PMID: 4295753
  17. Direct sequence determination of ovine luteinizing hormone releasing factor by mass spectrometry.
    Biochemistry. 1973 Dec 18;12(26):5305-10 PMID: 4586516
  18. Primary structure of the ovine hypothalamic luteinizing hormone-releasing factor (LRF) (LH-hypothalamus-LRF-gas chromatography-mass spectrometry-decapeptide-Edman degradation).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):278-82 PMID: 4550508
  19. Primary structure of somatostatin, a hypothalamic peptide that inhibits the secretion of pituitary growth hormone.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):684-8 PMID: 4514982
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.11.3942
Published
1976-11-00
Pages
3942-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431275
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.11.3942 PMC Full Text View journal details
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