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PMID: 10678979 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bacterial induction of beta interferon in mice is a function of the lipopolysaccharide component.

Infection and immunity ·Vol. 68 ·No. 3 ·2000-03-00 ·Pages 1600-7

Sing A, Merlin T, Knopf HP, Nielsen PJ, Loppnow H, Galanos C, Freudenberg MA

Abstract

We investigated the reason for the inability of lipopolysaccharide (LPS)-resistant (Lps-defective [Lps(d)]) C57BL/10ScCr mice to produce beta interferon (IFN-beta) when stimulated with bacteria. For this purpose, the IFN-beta and other macrophage cytokine responses induced by LPS and several killed gram-negative and gram-positive bacteria in LPS-sensitive (Lps-normal [Lps(n)]; C57BL/10ScSn and BALB/c) and Lps(d) (C57BL/10ScCr and BALB/c/l) mice in vitro and in vivo were investigated on the mRNA and protein levels. In addition, double-stranded RNA (dsRNA) was used as a nonbacterial stimulus. LPS and all gram-negative bacteria employed induced IFN-beta in the Lps(n) mice but not in the Lps(d) mice. All gram-positive bacteria tested failed to induce significant amounts of IFN-beta in all four of the mouse strains used. As expected, all other cytokines tested (tumor necrosis factor alpha, interleukin 1alpha [IL-1alpha], IL-6, and IL-10) were differentially induced by gram-negative and gram-positive bacteria. Stimulation with dsRNA induced IFN-beta and all other cytokines mentioned above in all mouse strains, regardless of their LPS sensitivities. The results suggest strongly that LPS is the only bacterial component capable of inducing IFN-beta in significant amounts that are readily detectable under the conditions used in this study. Consequently, in mice, IFN-beta is inducible only by gram-negative bacteria, but not in C57BL/10ScCr or other LPS-resistant mice.

MeSH Terms
Animals Cytokines/biosynthesis Female Gram-Negative Bacteria/physiology Gram-Positive Bacteria/physiology Interferon-alpha/biosynthesis Interferon-beta/biosynthesis,genetics Interferon-gamma/biosynthesis Interleukin-6/genetics Lipopolysaccharides/toxicity Macrophages/metabolism Male Mice Mice, Inbred BALB C Mice, Inbred C57BL RNA, Messenger/analysis Tumor Necrosis Factor-alpha/genetics
Chemicals
Cytokines Interferon-alpha Interleukin-6 Lipopolysaccharides RNA, Messenger Tumor Necrosis Factor-alpha Interferon-beta Interferon-gamma
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sing A
Max-Planck-Institut für Immunbiologie, D-79108 Freiburg, Germany.
Merlin T
Knopf H P
Nielsen P J
Loppnow H
Galanos C
Freudenberg M A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.3.1600-1607.2000
Published
2000-03-00
Pages
1600-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97320
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.68.3.1600-1607.2000 PMC Full Text View journal details
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