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PMID: 10677490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 4 ·2000-02-15 ·Pages 1501-5

Hajnsdorf E, Régnier P

Abstract

Current evidence suggests that the length of poly(A) tails of bacterial mRNAs result from a competition between poly(A) polymerase and exoribonucleases that attack the 3' ends of RNAs. Here, we show that host factor Hfq is also involved in poly(A) tail metabolism. Inactivation of the hfq gene reduces the length of poly(A) tails synthesized at the 3' end of the rpsO mRNA by poly(A) polymerase I in vivo. In vitro, Hfq stimulates synthesis of long tails by poly(A) polymerase I. The strong binding of Hfq to oligoadenylated RNA probably explains why it stimulates elongation of primers that already harbor tails of 20-35 A. Polyadenylation becomes processive in the presence of Hfq. The similar properties of Hfq and the PABPII poly(A) binding protein, which stimulates poly(A) tail elongation in mammals, indicates that similar mechanisms control poly(A) tail synthesis in prokaryotes and eukaryotes.

MeSH Terms
Carrier Proteins/genetics,metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Host Factor 1 Protein Integration Host Factors Kinetics Poly A/metabolism Polynucleotide Adenylyltransferase/metabolism RNA, Bacterial/genetics RNA, Messenger/genetics RNA-Binding Proteins/genetics Telomerase/metabolism
Chemicals
Carrier Proteins Escherichia coli Proteins Host Factor 1 Protein Integration Host Factors RNA, Bacterial RNA, Messenger RNA-Binding Proteins integration host factor, E coli Poly A Polynucleotide Adenylyltransferase Telomerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hajnsdorf E
Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France.
Régnier P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.040549897
Published
2000-02-15
Pages
1501-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26463
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.040549897 PMC Full Text View journal details
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