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PMID: 10673499 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Gene dose-dependent control of hematopoiesis and hematologic tumor suppression by CBP.

Genes & development ·Vol. 14 ·No. 3 ·2000-02-01 ·Pages 272-7

Kung AL, Rebel VI, Bronson RT, Ch'ng LE, Sieff CA, Livingston DM, Yao TP

Abstract

Mice with monoallelic inactivation of the CBP gene develop highly penetrant, multilineage defects in hematopoietic differentiation and, with advancing age, an increased incidence of hematologic malignancies. The latter are characterized, at least in some cases, by loss of heterozygosity (LOH) at the CBP locus. No such pathology was observed in wild-type or p300 heterozygous null mice of the same age and genetic background. Thus, a full complement of CBP, but not p300, is required for normal hematopoietic differentiation. These results also provide the first experimental evidence for the hypothesis that CBP has tumor-suppressing activity.

MeSH Terms
Animals Blotting, Southern Blotting, Western Bone Marrow Transplantation CREB-Binding Protein Cell Transplantation E1A-Associated p300 Protein Genes, Tumor Suppressor/genetics Hematologic Neoplasms/genetics,pathology Hematopoiesis/genetics Heterozygote Loss of Heterozygosity Mice Mice, Inbred C57BL Mice, Knockout Nuclear Proteins/genetics,metabolism Phenotype Spleen/cytology Trans-Activators/genetics,metabolism
Chemicals
Nuclear Proteins Trans-Activators CREB-Binding Protein Crebbp protein, mouse E1A-Associated p300 Protein Ep300 protein, mouse
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kung A L
Dana-Farber Cancer Institute, and Harvard Medical School, Boston, Massachusetts 02115, USA.
Rebel V I
Bronson R T
Ch'ng L E
Sieff C A
Livingston D M
Yao T P
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
Published
2000-02-01
Pages
272-7
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316359
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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