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PMID: 1066693 Published · ppublish English Journal Article

Decreased fidelity of DNA polymerase activity isolated from aging human fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 8 ·1976-08-00 ·Pages 2818-22

Linn S, Kairis M, Holliday R

Abstract

DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyltransferase, EC 2.7.7.7 or DNA nucleotidyltransferase) activity, isolated from late and early passage cells of the diploid human fibroblast line, MRC-5, was compared. The level of activity dropped with increasing passage. In addition, when the fidelity of polymerization was monitored with four synthetic templates under a variety of conditions, it was observed that the enzyme from late passage cells was more error-prone. The possible relation of these observations to "senescence" of the fibroblasts is discussed.

MeSH Terms
DNA Nucleotidyltransferases/metabolism DNA Replication Fibroblasts/enzymology Humans Manganese/pharmacology Templates, Genetic Time Factors
Chemicals
Manganese DNA Nucleotidyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Linn S
Kairis M
Holliday R
References (24)
24 references, click to expand
  1. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.
    Exp Cell Res. 1965 Mar;37:614-36 PMID: 14315085
  2. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.
    J Biol Chem. 1964 Jan;239:222-32 PMID: 14114848
  3. The maintenance of the accuracy of protein synthesis and its relevance to ageing.
    Proc Natl Acad Sci U S A. 1963 Apr;49:517-21 PMID: 13940312
  4. Enzymatic synthesis of deoxyribonucleic acid. X. Influence of bromouracil substitutions on replication.
    Proc Natl Acad Sci U S A. 1962 Mar 15;48:449-55 PMID: 13922323
  5. Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli.
    J Biol Chem. 1958 Jul;233(1):163-70 PMID: 13563462
  6. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  7. DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization.
    Biochem Biophys Res Commun. 1971 Aug 20;44(4):879-85 PMID: 5166448
  8. Premature ageing and occurance of altered enzyme in Werner's syndrome fibroblasts.
    Nature. 1974 Apr 26;248(5451):762-3 PMID: 4835548
  9. Inactive enzyme molecules in aging mice: liver aldolase.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):909-13 PMID: 4197629
  10. Replicative life-span of cultivated human cells. Effects of donor's age, tissue, and genotype.
    Lab Invest. 1970 Jul;23(1):86-92 PMID: 5431223
  11. DNA polymerases from human cells.
    Nat New Biol. 1971 Jun 9;231(23):167-70 PMID: 5281194
  12. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.
    Nature. 1975 Feb 6;253(5491):461-2 PMID: 46112
  13. Ageing of clones of mammalian cells.
    Nature. 1973 Jun 22;243(5408):441-5 PMID: 4591306
  14. Altered rate of DNA replication in ageing human fibroblast cultures.
    Nature. 1974 Oct 4;251(5474):434-6 PMID: 4423711
  15. An in vitro transversion by a mutationally altered T4-induced DNA polymerase.
    J Mol Biol. 1968 Sep 28;36(3):321-33 PMID: 4939629
  16. Low molecular weight DNA polymerase: decreased activity in spleens of old Balb/c mice.
    Mech Ageing Dev. 1975 Mar-Apr;4(2):123-36 PMID: 1152544
  17. A rapid rise in cell variants during the senescence of populations of human fibroblasts.
    Cell. 1975 Sep;6(1):67-73 PMID: 1164734
  18. Altered protein hypothesis of mammalian ageing processed-I. Thermal stability of glucose-6-phosphate dehydrogenase in C57BL/6J mouse tissue.
    Exp Gerontol. 1975 Apr;10(2):101-17 PMID: 1169161
  19. T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template.
    Biochem Biophys Res Commun. 1975 May 19;64(2):457-64 PMID: 1170851
  20. Heat-labile enzymes in skin fibroblasts from subjects with progeria.
    N Engl J Med. 1975 Jun 19;292(25):1305-9 PMID: 1128606
  21. Enzymatic synthesis of oligodeoxynucleotides.
    Biochemistry. 1971 Feb 2;10(3):536-42 PMID: 5543985
  22. Mistranslation and ageing in Neurospora.
    Nature. 1970 Nov 28;228(5274):877-80 PMID: 5477021
  23. Altered enzymes in ageing human fibroblasts.
    Nature. 1972 Jul 7;238(5358):26-30 PMID: 12635262
  24. Error theory and ageing in human diploid fibroblasts.
    Nature. 1972 Oct 6;239(5371):316-8 PMID: 12635218
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.8.2818
Published
1976-08-00
Pages
2818-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430760
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.8.2818 PMC Full Text View journal details
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