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PMID: 1065876 Published · ppublish English Journal Article

Temporal relationships of chromatin protein synthesis, DNA synthesis, and assembly of deoxyribonucleoprotein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2270-4

Seale RL

Abstract

Chromatin assembly has been investigated in terms of the sites on DNA where newly synthesized chromatin proteins associate. Chromatin from cells labeled with [14C]-BrdUrd and [3H]lysine was fixed with formaldehyde and resolved in CsCl gradients. By varying the spacing of the labeling intervals of the two isotopes so as to encompass all possible periods in S-phase, the association of labeled, newly synthesized proteins on newly synthesized (BrdUrd-substituted) or preexisting chromatin DNA was determined. In all experiments it was found that newly synthesized chromatin proteins predominantly associated with nonreplicating DNA. Possible mechanisms by which cells recycle preexisting chromatin proteins to restore the protein content of newly synthesized DNA are discussed.

MeSH Terms
Binding Sites Cell Division Chromatin/metabolism DNA/biosynthesis,metabolism DNA Replication Deoxyribonucleoproteins/metabolism HeLa Cells Histones/biosynthesis,metabolism Nucleoproteins/metabolism Time Factors
Chemicals
Chromatin Deoxyribonucleoproteins Histones Nucleoproteins DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Seale R L
References (36)
36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2270
Published
1976-07-00
Pages
2270-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430526
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2270 PMC Full Text View journal details
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